Anthropogenic disturbances pose a multitude of novel challenges to ecosystems. While many experiments have tested effects using abrupt treatment applications, most environmental changes in fact are gradual. Since ecosystem responses might be highly dependent on the temporal nature of stressors, it is crucial to differentiate the effects of abrupt vs gradual treatment application. Antifungal agents, which are widely used in disease control both for humans and in agriculture, are becoming a new class of environmental contaminants. In this study, we examined the effect of a sub-lethal application of one antifungal agent, amphotericin B. We applied different rates of delivery, e.g. gradual and abrupt, and monitored biomass and sporulation of the model fungus Neurospora crassa in a batch culture. Our results demonstrate that: (i) the effect size difference between abrupt and gradual treatments is fungal growth stage dependent and (ii) the gradual treatment clearly had a higher sporulation level compared with all types of abrupt treatments. Our findings highlight the importance of considering the rate of change in environmental change research and point to a new research direction for future global change studies. Furthermore, our results also have important implications for avoiding treatment-induced spore production in agriculture and medical practise.