Die Versorgung sterbender und verstorbener Patient*innen sowie die fürsorgende Auseinandersetzung mit Angehörigen verlangt von Ärzt*innen hohe Sensibilität für fremde und eigene Empfindungen und Bedürfnisse. Doch inwiefern beeinflusst die Konfrontation mit dem Tod den ärztlichen Umgang mit dem eigenen Lebensende? Wie und in welchen (Text-)Formen werden Alter(n), Sterben und Tod imaginiert und narrativiert? Und welche Bezüge werden zwischen dem fremden und dem eigenen Leben(-sende) hergestellt? Die Beiträger*innen suchen anhand von fiktionalen und faktualen Quellen nach Antworten auf diese Fragen und erschließen im interdisziplinären Feld der Medical Humanities den Umgang von Mediziner*innen mit der eigenen Sterblichkeit.

This article examines the processes involved in materializing the past. The recording of archaeological objects plays a pivotal role in establishing artefacts as valuable data that can be categorized, classified, and analysed to turn into historical narratives. It contributes significantly to shaping our understanding of the past: while conveying information about the objects themselves, this documentation inherently captures the subjective context of its recording and continues to influence our interpretations. In this article, both objective details and subjective conceptions are analysed from the records (drawings, photographs, reports) made at the rock figure of Karabel (Turkey), a monumental bas-relief discovered by European explorers in the 1830s. The author uses Karabel's diverse and controversial interpretations to examine how knowledge and ideas about the past evolve. To counterbalance the conventional linear interpretation of the past, he offers some insights into non-academic aspects of the monument.

Specialized or secondary metabolites are small molecules of biological origin, often showing potent biological activities with applications in agriculture, engineering and medicine. Usually, the biosynthesis of these natural products is governed by sets of co-regulated and physically clustered genes known as biosynthetic gene clusters (BGCs). To share information about BGCs in a standardized and machine-readable way, the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard and repository was initiated in 2015. Since its conception, MIBiG has been regularly updated to expand data coverage and remain up to date with innovations in natural product research. Here, we describe MIBiG version 4.0, an extensive update to the data repository and the underlying data standard. In a massive community annotation effort, 267 contributors performed 8304 edits, creating 557 new entries and modifying 590 existing entries, resulting in a new total of 3059 curated entries in MIBiG. Particular attention was paid to ensuring high data quality, with automated data validation using a newly developed custom submission portal prototype, paired with a novel peer-reviewing model. MIBiG 4.0 also takes steps towards a rolling release model and a broader involvement of the scientific community. MIBiG 4.0 is accessible online at https://mibig.secondarymetabolites.org/.

The AU Microscopii planetary system is only 24 Myr old, and its geometry may provide clues about the early dynamical history of planetary systems. Here, we present the first measurement of the Rossiter-McLaughlin effect for the warm sub-Neptune AU Mic c, using two transits observed simultaneously with the European Southern Observatory's (ESO's) Very Large Telescope (VLT)/Echelle SPectrograph for Rocky Exoplanets and Stable Spectroscopic Observations (ESPRESSO), CHaracterising ExOPlanet Satellite (CHEOPS), and Next-Generation Transit Survey (NGTS). After correcting for flares and for the magnetic activity of the host star, and accounting for transit-timing variations, we find the sky-projected spin-orbit angle of planet c to be in the range lambda(c) = 67.8(-49.0)(+31.7) degrees (1 sigma). We examine the possibility that planet c is misaligned with respect to the orbit of the inner planet b (lambda(b) = -2.96(-10.30)(+10.44)), and the equatorial plane of the host star, and discuss scenarios that could explain both this and the planet's high density, including secular interactions with other bodies in the system or a giant impact. We note that a significantly misaligned orbit for planet c is in some degree of tension with the dynamical stability of the system, and with the fact that we see both planets in transit, though these arguments alone do not preclude such an orbit. Further observations would be highly desirable to constrain the spin-orbit angle of planet c more precisely.

Context. AU Mic is a very active M dwarf star with an edge-on debris disk and two known transiting sub-Neptunes with a possible third planetary companion. The two transiting planets exhibit significant transit-timing variations (TTVs) that are caused by the gravi tational interaction between the bodies in the system.

Aims. Using photometrical observations taken with the CHaracterizing ExOPlanet Satellite (CHEOPS), we aim to constrain the plan etary radii, the orbital distances, and the periods of AU Mic b and c. Furthermore, our goal is to determine the superperiod of the TTVs for AU Mic b and to update the transit ephemeris for both planets. Additionally, based on the perceived TTVs, we study the possible presence of a third planet in the system.

Methods. We conducted ultra-high precision photometric observations with CHEOPS in 2022 and 2023. We used Allesfitter to fit the planetary transits and to constrain the planetary and orbital parameters. We combined our new measurements with results from previous years to determine the periods and amplitudes of the TTVs. We applied dynamical modelling based on TTV measurements from the 2018–2023 period to reconstruct the perceived variations.

Results. We found that the orbital distances and periods for AU Mic b and c agree with the results from previous works. However, the values for the planetary radii deviate slightly from previous values, which we attribute to the effect of spots on the stellar surface. AU Mic c showed very strong TTVs, with transits that occurred ∼80 minutes later in 2023 than in 2021. Through a dynamical analysis of the system, we found that the observed TTVs can be explained by a third planet with an orbital period of ∼12.6 days and a mass of 0.203−0.024+0.022 M⊕. We explored the orbital geometry of the system and found that AU Mic c has a misaligned retrograde orbit. The limited number of AU Mic observations prevented us from determining the exact dynamical configuration and planetary parameters. Further monitoring of the system with CHEOPS might help to improve these results.

We present new observations from CHEOPS (CHaracterising ExOPlanet Satellite) and TESS (Transiting Exoplanet Survey Satellite) to clarify the architecture of the planetary system hosted by the old Galactic thick disc star TOI-561. Our global analysis, which also includes previously published photometric and radial velocity data, incontrovertibly proves that TOI-561 is hosting at least four transiting planets with periods of 0.44 d (TOI-561 b), 10.8 d (TOI-561 c), 25.7 d (TOI-561 d), and 77.1 d (TOI-561 e) and a fifth non-transiting candidate, TOI-561f with a period of 433 d. The precise characterization of TOI-561's orbital architecture is interesting since old and metal-poor thick disc stars are less likely to host ultrashort-period super-Earths like TOI-561 b. The new period of planet -e is consistent with the value obtained using radial velocity alone and is now known to be 77.14399 +/- 0.00025 d, thanks to the new CHEOPS and TESS transits. The new data allowed us to improve its radius (R-p = 2.517 +/- 0.045 R-circle plus from 5 per cent to 2 per cent precision) and mass (M-p = 12.4 +/- 1.4 M-circle plus) estimates, implying a density of rho(p) = 0.778 +/- 0.097 R-circle plus. Thanks to recent TESS observations and the focused CHEOPS visit of the transit of TOI-561 e, a good candidate for exomoon searches, the planet's period is finally constrained, allowing us to predict transit times through 2030 with 20-min accuracy. We present an updated version of the internal structure of the four transiting planets. We finally performed a detailed stability analysis, which confirmed the long-term stability of the outer planet TOI-561 f.

Although the intraoperative molecular diagnosis of the approximately 100 known brain tumor entities described to date has been a goal of neuropathology for the past decade, achieving this within a clinically relevant timeframe of under 1 h after biopsy collection remains elusive. Advances in third-generation sequencing have brought this goal closer, but established machine learning techniques rely on computationally intensive methods, making them impractical for live diagnostic workflows in clinical applications. Here we present MethyLYZR, a naive Bayesian framework enabling fully tractable, live classification of cancer epigenomes. For evaluation, we used nanopore sequencing to classify over 200 brain tumor samples, including 10 sequenced in a clinical setting next to the operating room, achieving highly accurate results within 15 min of sequencing. MethyLYZR can be run in parallel with an ongoing nanopore experiment with negligible computational overhead. Therefore, the only limiting factors for even faster time to results are DNA extraction time and the nanopore sequencer’s maximum parallel throughput. Although more evidence from prospective studies is needed, our study suggests the potential applicability of MethyLYZR for live molecular classification of nervous system malignancies using nanopore sequencing not only for the neurosurgical intraoperative use case but also for other oncologic indications and the classification of tumors from cell-free DNA in liquid biopsies.

Microbial carbon use efficiency (CUE) is a key microbial trait affecting soil organic carbon (SOC) dynamics. However, we lack a unified and predictive understanding of the mechanisms underpinning the temperature response of microbial CUE, and, thus, its impacts on SOC storage in a warming world. Here, we leverage three independent soil datasets (n = 618 for microbial CUE; n = 591 and 660 for heterotrophic respiration) at broad spatial scales to investigate the microbial thermal response and its implications for SOC responses to warming. We show a nonlinear increase and decrease of CUE and heterotrophic respiration, respectively, in response to mean annual temperature (MAT), with a thermal threshold at ≈15 °C. These nonlinear relationships are mainly associated with changes in the fungal-to-bacterial biomass ratio. Our microbial-explicit SOC model predicts significant SOC losses at MAT above ≈15 °C due to increased CUE, total microbial biomass, and heterotrophic respiration, implying a potential abrupt transition to more vulnerable SOC under climate warming.

Red teaming, the practice of adversarially exposing unexpected or undesired model behaviors, is critical towards improving equity and accuracy of large language models, but non-model creator-affiliated red teaming is scant in healthcare. We convened teams of clinicians, medical and engineering students, and technical professionals (80 participants total) to stress-test models with real-world clinical cases and categorize inappropriate responses along axes of safety, privacy, hallucinations/accuracy, and bias. Six medically-trained reviewers re-analyzed prompt-response pairs and added qualitative annotations. Of 376 unique prompts (1504 responses), 20.1% were inappropriate (GPT-3.5: 25.8%; GPT-4.0: 16%; GPT-4.0 with Internet: 17.8%). Subsequently, we show the utility of our benchmark by testing GPT-4o, a model released after our event (20.4% inappropriate). 21.5% of responses appropriate with GPT-3.5 were inappropriate in updated models. We share insights for constructing red teaming prompts, and present our benchmark for iterative model assessments.

Arbuscular mycorrhizal (AM) fungi (phylum Glomeromycota) are obligate symbionts with plants influencing plant health, soil a(biotic) processes, and ecosystem functioning. Despite advancements in molecular techniques, understanding the role of AM fungal communities on a(biotic) processes based on AM fungal taxonomy remains challenging. This review advocates for a standardized trait-based framework to elucidate the life-history traits of AM fungi, focusing on their roles in three dimensions: host plants, soil, and AM fungal ecology. We define morphological, physiological, and genetic key traits, explore their functional roles and propose methodologies for their consistent measurement, enabling cross-study comparisons towards improved predictability of ecological function. We aim for this review to lay the groundwork for establishing a baseline of AM fungal trait responses under varying environmental conditions. Furthermore, we emphasize the need to include underrepresented taxa in research and utilize advances in machine learning and microphotography for data standardization.

Este libro recorre la historia de la Policía, el delito y los delincuentes en Colombia entre mediados del siglo XIX y principios del XX. A través del estudio de las ciencias policiales y sus sistemas de identificación criminal – la antropometría y la fotografía – se analizan las complejas relaciones entre el Estado y la sociedad; los cuerpos de vigilancia y la criminalidad urbana y rural; los saberes globales y locales; las conexiones entre arte y ciencia, así como los conflictos urbanos, electorales e intelectuales de la época. El texto se enfoca en el proceso de circulación global y apropiación local de la antropometría criminal, conocida como Bertillonage, dentro de la Policía Nacional como parte de su agenda reformista a inicios del siglo XX. Este período fue clave en la consolidación de la antropometría como una herramienta fundamental para el mantenimiento del orden social, gracias a su capacidad de individualizar reincidentes y fortalecer la operatividad policial. Este proceso no exento de resistencias. Cuadrillas de malhechores, revueltas artesanales y obreras, insurrecciones populares, atentados, magnicidios, hurtos, prostitución, apuñalamientos y la llegada de criminales internacionales no solo evidencian la evolución paralela y dialéctica del delito y de los saberes policiales, sino que también reflejan las resistencias “desde abajo” a las prácticas de vigilancia e identificación. Estas manifestaciones permiten una lectura más allá de los discursos oficiales, tradicionalmente centrados en la efectividad y el control social. Desde una perspectiva subalterna, el libro examina los efectos de las ciencias policiales sobre el honor y la identidad de quienes fueron sometidos a sus métodos de identificación, revelando las tensiones entre seguridad, control y derechos individuales.

Gegenstand des Forschungsprojektes war die Präsenz der theologischen Philosophie und der philosophischen Theologie im patristischen Denken (Ost und West). Die patristischen Testimonien zum Corpus Hermeticum wurden in diesem Kontext erstmalig umfassend erforscht. Die systematische Analyse erstreckte sich auf folgende Autoren: Athenagoras von Athen, Clemens von Alexandria, Origenes von Alexandria, Firmianus Lactantius, Didymos von Alexandria (einschl. Pseudo-Didymos), Synesios von Kyrene und Kyrill von Alexandria. Die Auswertung der übrigen patristischen Testimonien über Hermes Trismegistos brachte eine Reihe weiterer neuer Ergebnisse zutage. Das Forschungsprojekt zeigte die wesentliche Teilhabe des Christentums an der kaiserzeitlichen Pluralisierung des Philosophiebegriffs am Beispiel der patristischen Auseinandersetzung mit den zwei genannten Denkformen auf. Durch das Forschungsprojekt ist die Wirkungsgeschichte des Corpus Hermeticum in der patristischen Tradition grundlegend aufgehellt worden.

Ergothioneine (ET), a dietary thione/thiol, is receiving growing attention for its possible benefits in healthy aging and metabolic resilience. Our study investigates ET’s effects on healthspan in aged animals, revealing lifespan extension and enhanced mobility in Caenorhabditis elegans, accompanied by improved stress resistance and reduced age-associated biomarkers. In aged rats, ET administration enhances exercise endurance, muscle mass, and vascularization, concomitant with higher NAD+ levels in muscle. Mechanistically, ET acts as an alternative substrate for cystathionine gamma-lyase (CSE), stimulating H2S production, which increases protein persulfidation of more than 300 protein targets. Among these, protein-persulfidation-driven activation of cytosolic glycerol-3-phosphate dehydrogenase (cGPDH) primarily contributes to the ET-induced NAD+ increase. ET’s effects are abolished in models lacking CSE or cGPDH, highlighting the essential role of H2S signaling and protein persulfidation. These findings elucidate ET’s multifaceted actions and provide insights into its therapeutic potential for combating age-related muscle decline and metabolic perturbations.

The effects of atmospheric aerosols are key uncertainties in climate models. One reason is the complex aerosol composition which includes a relatively large fraction of organics. Another reason is the small size of aerosols, which makes surface effects and processes important. These two factors make surface-active organics relevant for atmospheric aerosols, as they can affect crucial processes, such as chemical aging and water accommodation, as well as properties such as the surface tension, which drives droplet formation. Two exemplary types of atmospherically relevant organics are carboxylic acids and alkyl amines, and often both are found together within aerosols. In the most atmospherically significant pH range, these exist as alkyl-carboxylate ions and alkyl-ammonium ions. Using liquid-jet photoelectron spectroscopy, tuned to high surface sensitivity, we measured the alkyl-carboxylate anions and the alkyl-ammonium cations of alkyl chain lengths of 1 to 6 carbon atoms, both as single-component and mixed-component aqueous solutions. This enabled us to systematically study how their surface propensity is affected by the length of the alkyl chains and how cooperative ion–ion interactions result in strongly increased surface propensity. An exponential increase in surface propensity is found for the single-species solutions, with cooperative solute–solute effects in mixed solutions of 1 : 1 molar ratio drastically increasing the number of molecules present at the solutions' surfaces up to a factor of several hundred. This cooperative surface propensity is shown to strongly affect the amounts of organics at the surface. These changes can significantly influence radiative forcing via aerosol growth, cloud condensation nuclei activity, and aerosol chemical aging. Our results demonstrate the principal feasibility of a more advanced input of molecular details for creating parameterized descriptions of aerosol surface composition needed to properly account for their impacts in climate models.

Background

Childhood maltreatment (CM) significantly increases the risk of developing post-traumatic stress disorder (PTSD) for which the prevalence in Europe is higher than initially assumed. While the high economic burden of PTSD is well-documented, little is known about the health care cost differences between individuals with PTSD-CM and those without PTSD in Germany. This study aimed to determine the excess health care and absenteeism costs associated with PTSD-CM in Germany.

Methods

Baseline data from a multi-center randomized controlled trial on individuals with PTSD-CM (n = 361) were combined with data from individuals without PTSD (n = 4760). Entropy balancing was used to balance the data sets with regard to sociodemographic characteristics. Six-month excess health care costs from a societal perspective were calculated for 2022, using two-part models with logit specification for the first part and a generalized linear model for the second part.

Results

The total six-month excess costs associated with PTSD-CM were €8864 (95% CI: €6855 to €10,873) per person. Of this, the excess health care costs accounted for €4647 (95% CI €3296 to €5997) and the excess costs of absenteeism for €4217 (95% CI: €3121 to €5314). Individuals with mild to moderate PTSD symptoms incurred total excess costs of €6038 (95% CI: €3879 to €8197), while those with severe to extreme symptoms faced €11,433 (95% CI: €8220 to €14,646).

Conclusions

Excess health care and absenteeism costs associated with PTSD-CM were substantial, with absenteeism accounting for roughly half of the total excess costs.

Biginelli dihydropyrimidines (DHPMs) are considered superior over 1,4-dihydropyridines (DHPs) in terms of both light and metabolic stabilities. Nevertheless, DHPs dominate the market as the most prescribed calcium channel blockers with strong therapeutic potential in managing cardiovascular ailments. To overcome the restrictions that complicate the formulation and postadministration of DHPs, employing bioisosteric replacement by exchanging the DHP ring with DHPM appears as a logical approach for the improved formulations of new calcium channel blockers. In this study, we obtained DHPM derivatives via Biginelli synthesis and acetylated their N-3 position by heating them in acetic anhydride (GD1–GD12). We also incorporated the DHPM scaffold into a condensed ring system (GD13 and GD14). These DHPMs were evaluated for their ability to block both L- (Cav1.2) and T- (Cav3.2) type calcium channels. Compounds carrying acetyl moiety on the N-3 position of the DHPM scaffold appeared to be more effective inhibitors of both channels. Retesting GD4 enantiomers, separated using high-performance liquid chromatography (HPLC) on a chiral stationary phase, revealed that the (R)-isomer predominantly contributes to the outstanding inhibitory activity of GD4 on calcium channels. Molecular modeling studies, including docking, molecular dynamics simulations, and dynophore analysis, provided insights into the binding mechanism of DHPMs to Cav1.2 and Cav3.2, for the first time.

Magnetoelasticity plays a crucial role in numerous magnetic phenomena, including magnetocalorics, magnon excitation via acoustic waves, and ultrafast demagnetization, or the Einstein–de Haas effect. Despite a long-standing discussion on anisotropy-mediated magnetoelastic interactions of relativistic origin, the exchange-mediated magnetoelastic parameters within an atomistic framework have only recently begun to be investigated. As a result, many of their behaviors and values for real materials remain poorly understood. Therefore, by using a proposed simple modification of the embedded cluster approach that reduces the computational complexity, we critically analyze the properties of exchange-mediated spin-lattice coupling parameters for elemental 3⁢𝑑 ferromagnets (bcc Fe, fcc Ni, and fcc Co), comparing methods used for their extraction and relating their realistic values to symmetry considerations and orbitally decomposed contributions. Additionally, we investigate the effects of noncollinearity (spin temperature) and applied pressure on these parameters. For Fe, we find that single-site rotations, associated with spin temperatures around 100 K, induce significant modifications, particularly in Dzyaloshinskii-Moriya-type couplings; in contrast, such interactions in Co and Ni remain almost configuration independent. Moreover, we demonstrate a notable change in the exchange-mediated magnetoelastic constants for Fe under isotropic contraction. Finally, the conversion between atomistic, quantum-mechanically derived parameters and the phenomenological magnetoelastic theory is discussed, which can be a useful tool towards larger and more realistic dynamics simulations involving coupled subsystems.

Context. Recent developments in exoplanetary research highlight the importance of Love numbers in understanding the internal dynamics, formation, migration history, and potential habitability of exoplanets. Love numbers represent crucial parameters that gauge how exoplanets respond to external forces such as tidal interactions and rotational effects. By measuring these responses, insights into the internal structure, composition, and density distribution of exoplanets can be gained. The rate of apsidal precession of a planetary orbit is directly linked to the second-order fluid Love numbers. Thus, Love numbers can also offer valuable insights into the mass distribution of a planet.

Aims. In this context, we aim to re-determine the orbital parameters of WASP-43b – in particular, the orbital period, eccentricity, and argument of the periastron – and its orbital evolution. We study the outcomes of the tidal interaction with the host star in order to identify whether tidal decay and periastron precession occur in the system.

Methods. We observed WASP-43b with HARPS, whose data we present for the first time, and we also analysed the newly acquired JWST full-phase light curve. We jointly fit new and archival radial velocity and transit and occultation mid-times, including tidal decay, periastron precession, and long-term acceleration in the system.

Results. We detected a tidal decay rate of Ṗa = (−l.99±0.50) ms yr−1 and a periastron precession rate of ω = 0.1727−0.0089+0.0083)° d−1 = (621.72−32.04+29.88)″ d−1). This is the first time that both periastron precession and tidal decay are simultaneously detected in an exoplanetary system. The observed tidal interactions can neither be explained by the tidal contribution to apsidal motion of a non-aligned stellar or planetary rotation axis nor by assuming a non-synchronous rotation for the planet, and a value for the planetary Love number cannot be derived. Moreover, we excluded the presence of a second body (e.g. a distant companion star or a yet undiscovered planet) down to a planetary mass of ≳0.3 MJ and up to an orbital period of ≲3700 days. We leave the question of the cause of the observed apsidal motion open.

Methodological advances in seismic tomography are often driven by the quality of data sets. The dense and homogeneous spatial coverage of the AlpArray seismic network, including hundreds of permanent and temporary broadband stations, has motivated a series of methodological developments of ambient-noise-based tomography of the lithosphere across the entire Alps-Apennines regions, which have been published and are reviewed here. To take full advantage of the ocean-bottom seismometers (OBS) in the Ligurian-Provence basin, reconstructed Rayleigh wave signals between OBS have been improved by second-order correlations with onland stations. A Bayesian or fully transdimensional formalism has been introduced in both steps of isotropic ambient noise tomography. The three-dimensional S-wave velocity models have been further improved by wave-equation based inversions accounting for the physics of seismic wave propagation, including elastic–acoustic coupling at the sea bottom. A beamforming approach has been developed to avoid systematic errors in the measurement of azimuthal anisotropy from seismic noise. Probabilistic inversions for depth variations of azimuthal and radial anisotropy have provided robust estimates of anisotropic parameters in the crust and upper mantle that differ significantly from earlier surface-wave tomography studies. These methodological improvements have taken the full benefit of the quality of available seismic data to significantly improve knowledge of the seismic structure of the crust and shallow mantle beneath the Alps-Apennines system. Our findings include detailed mapping of strong and abrupt Moho depth changes under the Western Alps, contrasting orientations of fast velocity directions between the upper and lower Alpine crust, and the absence of significant radial anisotropy everywhere in the European crust and shallow upper mantle, except in the Apenninic lower crust. These methods can be applied to similar dense arrays with equivalent potential benefits.

This work was performed to generate the data needed to set epidemiological cut-off values for minimal inhibitory concentrations (MICs) of 10 antimicrobial agents against Vibrio parahaemolyticus determined using standardised broth microdilution protocols. Eight laboratories performed broth microdilution tests with incubation at 35°C for 16 to 20 h, and 7 also performed tests on the same isolates with incubation at 28°C for 24 to 28 h. Data were analysed by the ECOFFinder and normalised resistance interpretation algorithms. The cut-off values calculated for ceftazidime, florfenicol and trimethoprim/sulfamethoxazole, 1, 1 and 0.25/4.75 µg ml-1, respectively, were the same when calculated from data obtained at both temperatures. The cut-off values calculated from data obtained at 35°C and from data obtained at 28°C were 0.25 and 0.5 µg ml-1 for enrofloxacin, 2 and 4 µg ml-1 for gentamicin, 0.5 and 1 µg ml-1 for oxolinic acid and 2 and 1 µg ml-1 for oxytetracycline, respectively. The influence of incubation temperature on MIC values was investigated by comparing MICs obtained at 35 and 28°C for a specific antimicrobial agent with a particular isolate by an individual laboratory. Results showed that 56% of 1473 of these paired MIC values were identical, while 38% differed from one another by not more than 1 dilution step. The data generated in this work will be submitted to the Clinical and Laboratory Standards Institute for consideration in their setting of internationally agreed epidemiological cut-off values for V. parahaemolyticus that are essential for interpreting antimicrobial susceptibility testing data of this species.