Air pollution is increasing worldwide and skin is exposed to high levels of pollution daily, causing oxidative stress and other negative consequences. The methods used to determine oxidative stress in the skin are invasive and non-invasive label-free in vivo methods, which are severely limited. Here, a non-invasive and label-free method to determine the effect of cigarette smoke (CS) exposure on skin ex vivo (porcine) and in vivo (human) was established. The method is based on the measurement of significant CS-exposure-induced enhancement in red- and near-infrared (NIR)-excited autofluorescence (AF) intensities in the skin. To understand the origin of red- and NIR-excited skin AF, the skin was exposed to several doses of CS in a smoking chamber. UVA irradiation was used as a positive control of oxidative stress in the skin. The skin was measured with confocal Raman microspectroscopy before CS exposure, immediately after CS exposure, and after skin cleaning. CS exposure significantly increased the intensity of red- and NIR-excited skin AF in a dose-dependent manner in the epidermis, as confirmed by laser scanning microscopy AF imaging and fluorescence spectroscopy measurements. UVA irradiation enhanced the intensity of AF, but to a lower extent than CS exposure. We concluded that the increase in red- and NIR-excited AF intensities of the skin after CS exposure could clearly be related to the induction of oxidative stress in skin, where skin surface lipids are mainly oxidized.

There is extensive research on children’s intergroup attitudes, but their perceptions of refugee children have rarely been studied. We conducted a study with 5- and 6-year-old children (N = 60) in Germany following the arrival of unprecedented large numbers of refugees in 2015 and 2016. Children completed a set of three tasks that measured their perceptions of refugee children (minority group) and German children (majority group): a draw-a-typical-child task (including questions about whether participants wanted to interact with the depicted child), an intergroup attitude task, and a liking task. Results indicate that participants drew similar pictures of and had similar intentions to interact with refugee children and German children. There was mixed evidence for group favouritism: while participants showed similar explicit attitudes towards German and refugee peers, they indicated more liking of German peers. Moreover, children viewed refugee children as a less variable (more homogeneous) group than German children. Opportunities for intergroup contact with refugee peers (i.e., whether participants attended kindergartens with or without refugee children) had no discernible effect on any of the measures. Our findings provide a snapshot of children’s perceptions of refugees in a unique historical context and contribute to research on the development of intergroup attitudes in real-world settings.

The catalytic construction of well-defined materials from mixtures of building blocks is an important challenge in sustainable catalysis. In this regard, we have recently reported a new type of selective ring-opening terpolymerisation (ROTERP), in which three monomers (A, B, C) are selectively enchained into a (ABA′C)n sequence, but the reasons behind this unusual selectivity remained unanswered. In this study, we present a detailed investigation into the full ROTERP mechanism based on the reactivity of model intermediates, computational studies investigating >100 possible intermediates and transition states and reaction kinetics. Experimental verification of the intermediate speciation, the primary insertion steps and the side-reactions lets us show that although most insertions and side-reactions are thermodynamically viable, kinetic selection processes at the propagating chain end determine the sequence selectivity. Computational studies elucidate the special role and speciation of the lithium catalyst which during the catalytic cycle involves mono-metallic, bi-metallic and charge separated transition states comprising both coordinative activation of incoming monomers and functional groups of the polymer backbone adjacent to the propagating chain. Our study not only deciphers the mechanism of a rare selective terpolymerisation but also helps answering open questions relevant to ring-opening copolymerisation (ROCOP) and alkali-metal catalysis in general, thus guiding the design of future polymerisation catalysis for degradable materials.

Previous studies on the neuro-cognition of language have provided a strong case for systematic inter-individual variability in event-related potentials (ERPs) evoked during language processing. In the present study, we aimed at extending this evidence to the processing of morphologically complex words. We focused on German plural forms and tested two types of morphological violations: overapplications of regular plural morphemes (‘regularizations’) and of irregular plural morphemes (‘irregularizations’). The group-level results showed a biphasic LAN-P600 response for regularizations, and a P600 for irregularizations. In line with previous reports, our analyses of inter-individual variability suggested that biphasic responses consisting of a negativity followed by a positivity are unlikely to exist at the individual level. Importantly, when analyzing the scalp distribution of ERPs elicited in participants supposed to show negativity-dominant responses, we found this to vary as a function of the type of morphological form: regularizations elicited a left-hemisphere response (LAN), while irregularizations a more widespread negativity (N400). Our results are consistent with dual-route accounts of morphological processing that distinguish between rule-based processing for regular inflection and memory retrieval for irregular inflection. At a more general level, our study shows that complementing group-level results with analyses of inter-individual variability can crucially contribute to a more detailed understanding of brain signatures of language.

The article deals with the theatre parcours Kahvehane – Turkish Delight, German Fright?, a walking performance that takes the audience to various Anatolian coffee houses in the Berlin districts Kreuzberg and Neukölln. Within these venues, international artists developed performative interventions trying to temporarily open these seemingly closed spaces to a new (theatre) audience. By leaving the theater building and entering the Kahvehane, however, the audience itself became an object of investigation. As the essay attempts to show, the parcours stages aesthetic as well as social tensions between self and other, between the known and the unknown by deploying different medial strategies, as it consists not only of the tour, but also of a catalog including a cartography and a typology of the Kahvehane. However, the documenting map and the ephemeral (theater) parcours are not presented as mutually exclusive strategies, but as media practices that necessarily complement each other in order to negotiate and historicize conf licts of the German migration society with performative means.

In Europe, equines destined for human consumption (hereafter called slaughter equines) are subject to the same restrictions of usage of veterinary drugs as other food-producing animals, with amendments regulated in the so-called ‘positive list’, Regulation (EC) No. 1950/2006. Due to the complex legal requirements for drug administration in slaughter equines, it might be that specific knowledge regarding the legislation of slaughter equines may be insufficient among veterinarians, equine owners, and equine keepers. To study this assumption, three target group-specific surveys were conducted in 2021. Answers from 153 equine treating veterinarians, 170 equine owners, and 70 equine keepers were included in the analysis. In total 68.4% (91/133) of the participating veterinarians, the regulations of the ‘positive list’, Regulation (EC) No. 1950/2006, were ‘rather complicated’ to ‘complicated’. Among the participating veterinarians, 38.4% (58/151) did not or could not answer correctly how to proceed if a slaughter equine is scheduled to receive phenylbutazone, usage of which is prohibited in all livestock by Regulation (EU) No. 37/2010. Simultaneously, 56.2% (86/153) of the participating veterinarians named phenylbutazone as the, or one of the, most often used non-steroidal anti-inflammatory drugs. Altogether, 41.2% (70/170) of participating equine owners and 42.9% (30/70) of equine keepers did not know under which circumstances an equine can legally be slaughtered for human consumption. In total, 34.3% (24/70) of the equine keepers classified their knowledge of national regulations for animal keepers regarding the documentation of drug usage in equines as ‘poor’ to ‘nonexistent’. This lack of knowledge in all three surveyed groups, combined with the complex legal regulations regarding the usage and documentation of drugs in slaughter equines, could result in missing and false documentation, treatment of slaughter equines with prohibited substances and therefore pose a risk factor for drug residues in equine meat.

In the divided Berlin of the 1960s to 1980s, the S-Bahn held a specific position: it was object of controversial political debates, setting for numerous migration attempts, and of ten addressed as a metonymic representation of the (divided) urban architecture that was specific to Berlin. Thus it served as an important medium of ref lection for a number of authors, especially from the eastern part of the city: this article primarily examines poetry, essay writing, and prose texts by the former GDR authors Uwe Johnson, Günter Kunert, Fritz Rudolf Fries, Elke Erb, Johannes Jansen, and Annett Gröschner. Special attention needs to be payed to the S-Bahn as a poetic instrument for identifying an own position within the multi-layered Berlin topography: between East and West, affiliation and rejection, collective urban identity and self-assertion, performed and hindered border crossings.

This article traces different literary ways of engaging with Berlin’s historical topographies in works by Emine Sevgi Özdamar, Aras Ören and Tomer Gardi. It regards their texts as being an integral part of the city in an on-going dialogic process. This process is deeply interwoven with the poetics of each of the authors. The concept of affective topographies proposes to think of this relation as a dynamic of affecting and being-affected. In this view, affective topographies are explicitly including historical layers, temporalities as well as ›memory gaps‹. Reading Özdamar, Ören and Gardi alongside each other, the article lines out how the texts are linked and overlap, especially with regard to Berlin time-spaces. This dialogical reading is underscoring that Berlin’s topographies as well as Berlin’s literature are social phenomena constitutively shaped and formed by processes of (post-)migration. It sets out to provide a first sketch of what could be a literary history of Berlin within the horizon of postmigrant society.

Jenny Erpenbeck’s novel Go, Went, Gone portrays Berlin, the highly present city in the author’s work, as a city filled with histories and memories, which make up the city’s physical and mental (visible and invisible) layers. Analyses of this novel have so far centered on memory aspects, which implies a certain notion of permanence. This article turns towards the motif of impermanence and shows its predominance in Erpenbeck’s work. By scrutinizing the texture of the specific places in Berlin, this topographical reading aims to show how the places’ structure is shaped by a permanent impermanence. As these continuous transformations can be observed on different levels, the article unfolds a multilayered impermanence of Berlin.

The discovered light modulation capabilities of diatom silicious valves make them an excellent toolkit for photonic devices and applications. In this work, a reproducible surface-enhanced Raman scattering (SERS) enhancement was achieved with hybrid substrates employing diatom silica valves coated with an ultrathin uniform gold film. Three structurally different hybrid substrates, based on the valves of three dissimilar diatom species, have been compared to elucidate the structural contribution to SERS enhancement. The comparative analysis of obtained results showed that substrates containing cylindrical Aulacoseira sp. valves achieved the highest enhancement, up to 14-fold. Numerical analysis based on the frequency domain finite element method was carried out to supplement the experimental results. Our results demonstrate that diatom valves of different shapes can enhance the SERS signal, offering a toolbox for SERS-based sensors, where the magnitude of the enhancement depends on valve geometry and ultrastructure.

Phenotypic susceptibility testing of Escherichia (E.) coli is an essential tool to gain a better understanding of the potential impact of biocide selection pressure on antimicrobial resistance. We, therefore, determined the biocide and antimicrobial susceptibility of 216 extended-spectrum β-lactamase-producing (ESBL) and 177 non-ESBL E. coli isolated from swine feces, pork meat, voluntary donors and inpatients and evaluated associations between their susceptibilities. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of benzalkonium chloride, chlorhexidine digluconate (CHG), chlorocresol (PCMC), glutaraldehyde (GDA), isopropanol (IPA), octenidine dihydrochloride and sodium hypochlorite (NaOCl) showed unimodal distributions, indicating the absence of bacterial adaptation to biocides due to the acquisition of resistance mechanisms. Although MIC95 and MBC95 did not vary more than one doubling dilution step between isolates of porcine and human origin, significant differences in MIC and/or MBC distributions were identified for GDA, CHG, IPA, PCMC and NaOCl. Comparing non-ESBL and ESBL E. coli, significantly different MIC and/or MBC distributions were found for PCMC, CHG and GDA. Antimicrobial susceptibility testing revealed the highest frequency of resistant E. coli in the subpopulation isolated from inpatients. We observed significant but weakly positive correlations between biocide MICs and/or MBCs and antimicrobial MICs. In summary, our data indicate a rather moderate effect of biocide use on the susceptibility of E. coli to biocides and antimicrobials.

We prove that maximum a posteriori estimators are well-defined for diagonal Gaussian priors ,u on tp under common assumptions on the potential F. Further, we show connections to the Onsager-Machlup functional and provide a corrected and strongly simplified proof in the Hilbert space case p= 2, previously established by Dashti et al (2013 Inverse Problems 29 095017); Kretschmann (2019 PhD Thesis). These corrections do not generalize to the setting 1 ? p < 8, which requires a novel convexification result for the difference between the Cameron-Martin norm and the p-norm.

The known host range of circoviruses is continuously expanding because of more intensive diagnostic activities and advanced sequencing tools. Recently, a new circovirus (penguin circovirus (PenCV)) was identified in the guano and cloacal samples collected from Adélie penguins (Pygoscelis adeliae) and chinstrap penguins (Pygoscelis antarcticus) in Antarctica. Although the virus was detected in several asymptomatic subjects, a potential association with feather disease was speculated. To investigate the occurrence and implications of PenCV in other penguin species located outside of Antarctica, a broad survey was undertaken in African penguins (Spheniscus demersus) on two islands off the southern Namibian coast. For this purpose, specific molecular biology assays were developed and validated. None of the 151 blood samples tested positive for PenCV. Several reasons could explain the lack of PenCV positive samples. African penguins and Pygoscelis species are separated by approximately 6000 km, so there is almost no opportunity for transmission. Similarly, host susceptibility to PenCV might be penguin genus-specific. Overall, the present study found no evidence of PenCV in African penguin colonies in Namibia. Further dedicated studies are required to assess the relevance of PenCV among different penguin species.

Zwitterionic compounds such as pyridine-containing tellurenyl compounds are interesting building blocks for heterometallic assemblies. They can act as ambiphilic donor/acceptors as is shown by the products of reactions of the zwitterions HpyTeCl2 or HCF3pyTeCl2 with the rhenium(V) complex [ReOCl3(PPh3)2]. The products have a composition of [ReO2Cl(pyTeCl)(PPh3)2] and [ReO2Cl(CF3pyTeCl)(PPh3)2] with central {O=Re=O…Te(Cl)p𝑦}+ units. The Re-O bonds in the products are elongated by approximately 0.1 Å compared with those to the terminal oxido ligands and establish Te…O contacts. Thus, the normally easily assigned concept of oxidation states established at the two metal ions becomes questionable (ReV/TeII vs. ReIII/TeIV). A simple bond length consideration rather leads to a description with the coordination of a mesityltellurenyl(II) chloride unit to an oxido ligand of the Re(V) center, but the oxidation of the tellurium ion and the formation of a tellurinic acid chloride cannot be ruled out completely from an analysis of the solid-state structures. DFT calculations (QTAIM, NBO analysis) give clear support for the formation of a Re(V) dioxide complex donating into an organotellurium(II) chloride and the alternative description can at most be regarded as a less favored resonance structure.

Hard ticks pose a threat to animal and human health. Active life stages need to feed on a vertebrate host in order to complete their life cycle. To study processes such as tick-pathogen interactions or drug efficacy and pharmacokinetics, it is necessary to maintain tick colonies under defined laboratory conditions, typically using laboratory animals. The aim of this study was to test a membrane-based artificial feeding system (AFS) applicable for Amblyomma ticks using Amblyomma tonelliae as a biological model. Adult ticks from a laboratory colony were fed in a membrane-based AFS. For comparison, other A. tonelliae adults were fed on calf and rabbit. The proportions of attached (AFS: 76%; calf/rabbit: 100%) and engorged females (AFS: 47.4%; calf/rabbit: 100%) in the AFS were significantly lower compared to animal-based feeding (p = 0.0265). The engorgement weight of in vitro fed ticks (𝑥̲ = 658 mg; SD ± 259.80) did not significantly differ from that of ticks fed on animals (p = 0.3272, respectively 0.0947). The proportion of females that oviposited was 100% for all three feeding methods. However, the incubation period of eggs (𝑥̲ = 54 days; SD ± 7) was longer in the AFS compared to conventional animal-based feeding (p = 0.0014); 𝑥̲ = 45 days; SD ± 2 in the rabbit and (p = 0.0144). 𝑥̲ = 48 days; SD ± 2 in the calf). Egg cluster hatching (𝑥̲ = 41%; SD ± 44.82) was lower in the AFS than in the other feeding methods (rabbit: 𝑥̲ = 74%; SD ± 20; p = 0.0529; calf: 𝑥̲ = 81%; SD ± 22; p = 0.0256). Although the attachment, development, and the hatching of AFS ticks were below those from animal-based feeding, the method may be useful in future experiments. Nevertheless, further experiments with a higher number of tick specimens (including immature life stages) and different attractant stimuli are required to confirm the preliminary results of this study and to evaluate the applicability of AFS for Amblyomma ticks as an alternative to animal-based feeding methods.

Little is known about the animal- and diet-related factors that could interfere with the plasma zinc (Zn) concentrations of equines. Additionally, the adequacy of plasma to reflect changes in the Zn intake is unclear. In the first part of this study, the plasma Zn concentrations of hospitalized horses and ponies (n = 538) were measured and evaluated for the impact of the age, sex, horse type, and internal diseases of the animals. In the second part, the effects of increasing dietary Zn chloride hydroxide and Zn methionine supplementations were assessed on the plasma and mane hair Zn concentrations of healthy horses (n = 2) and ponies (n = 8). Part 1: The age, sex, and horse type did not influence the plasma Zn concentrations. No effect of internal diseases was observed, with the exception of higher plasma Zn concentrations in animals with metabolic disorders compared to the control group (p < 0.05). Part 2: Both Zn supplements dose-dependently increased the Zn concentrations in the mane hair (p = 0.003), but not in the plasma of the horses and ponies. In conclusion, the plasma Zn concentrations were widely unaffected by nutritional and non-nutritional factors in equines, while mane hair samples better reflected the dietary Zn supply.

This study investigates the socio-economic characteristics, behavioral preferences, and consumption of individuals who own crypto-assets. Our empirical analysis utilizes data from a German personal finance management app where users connect their bank accounts and depots. We conducted a survey and elicited behavioral factors for financial decision-making. By combining survey with account and security account data, we identify crypto investors’ preferences for financial decision-making and financial advice. Our results suggest that, in particular, students or self-employed, young, and male individuals who are risk-seeking and impatient are more likely to have invested in crypto-assets. Most crypto owners have less experience with financial advisory. They see it as too time-consuming and qualitatively poor, and instead, they prefer to decide on their own as they have self-reported high financial literacy. Investigating their consumption in more detail we conclude that crypto investors more often spend on travelling, electronics, and food delivery and less on health. Our findings suggest policymakers in identifying high-risk consumers and investors, and help financial institutions develop appropriate products.

Background: The antiseptic agent octenidine dihydrochloride (OCT) is used for skin preparation, for Staphylococcus aureus decolonization, and within bundles for the prevention of catheter-related or surgical site infections (SSIs). Here, we review the evidence for the effects of OCT from clinical studies. Methods: Review of studies published in the Medline, Scopus, and Cochrane databases until August 2022, performed in clinical settings and reporting on effects of OCT on S. aureus carriage/transmission, SSI prevention, and prevention of intensive care unit (ICU)-related or catheter-related bloodstream and insertion site infections. Results: We included 31 articles. The success of S. aureus decolonization with OCT-containing therapies ranged between 6 and 87%. Single studies demonstrated that OCT application led to a reduction in S. aureus infections, acquisition, and carriage. No study compared OCT for skin preparation before surgical interventions to other antiseptics. Weak evidence for the use of OCT for pre-operative washing was found in orthopedic and cardiac surgery, if combined with other topical measures. Mostly, studies did not demonstrate that daily OCT bathing reduced ICU-/catheter-related bloodstream infections with one exception. Conclusions: There is a need to perform studies assessing the clinical use of OCT compared with other antiseptics with respect to its effectiveness to prevent nosocomial infections.