Groupwise antibiotic treatments are common in broiler chicken production. They induce selection for antibiotic resistance in commensalEscherichia coli.This study aimed to investigate antibiotic resistance after individual (I, drenching) or groupwise treatment (G, by water) with amoxicillin, and after contact with I or G (KI or KG), compared with untreated broilers without contact with treated broilers (C), and pretreatment values. Finally, we compared antibiotic resistance from broilers (G) after a second treatment, with a treatment in the contact animals (KG), and a first treatment in the control animals (C). Resistance to ampicillin and other antibiotics was significantly increased in groups G and I within 2 days, suggesting (co-)selection of resistance. The increase was lower in groups KI, KG, and C during the first treatment (days 1-5). The increased resistance in group C was interpreted as a change in the microbiota after initial moving and first feeding. After treatment, resistance rates decreased to initial or lower values in all groups. During the second treatment period (days 34-38), all three groups' (G, KG, and C) resistance levels increased to equally high levels. Cephalosporin resistance was low, and did not change over the experimental period. On days 3 and 38, resistance rates ofE. colifrom duodenum, jejunum, and cecum did not differ between segments and treatment routes. Overall, the baseline levels of antibiotic resistance inE. coliwere high. Amoxicillin triggered an increase in resistance levels, irrespective of the mode of treatment. Substantial resistance dynamics in untreated controls warrant further investigation.View less
Brucellosis is reportedly endemic in ruminants in Pakistan. BothBrucella abortusandB. melitensisinfections have been decumented in domestic animals and humans in the country. This study aimed to identify the burden of anti-Brucellaantibodies in small ruminants as well as associated potential risk factors with its occurrence at nine institutional livestock farms in Punjab, Pakistan. The sera collected from equal number of sheep and goats (500 from each species) were screened by indirect-ELISA for anti-smooth-Brucellaantibodies followed by a serial detection by real-time PCR. Overall, 5.1% (51/1000) seropositivity was registered corresponding to 5% (25/500) prevalence in goats and 5.2% (26/500) in sheep.Brucella-DNA could not be detected in any of the tested sera by real-time PCR. Multiple logistic regression model indicated that farm location (OR 34.05), >4 years of age (OR 2.88), with history of reproductive disorders (OR 2.69), and with BCS of <= 3 (OR 12.37) were more likely to test positive for brucellosis at these farms. A routine screening, stringent biosecurity, and quarantine measures are warranted for monitoring and eradication of the infection. Similarly, isolation and molecular investigation of the etiologic agent(s) are needed to understand the relationship of epidemiology and out-breaks of brucellosis in the country.View less
The reliability of transferring species distribution models (SDMs) to new ranges and future climates has been widely debated. Biological invasions offer the unique opportunity to evaluate model transferability, as distribution data between species' native and introduced ranges are geographically independent of each other. Here, we performed the first global quantitative synthesis of the spatial transferability of SDMs for 235 invasive species and assessed the association of model transferability with the focal invader, model choice and parameterisation. We found that SDMs had limited spatial transferability overall. However, model transferability was higher for terrestrial endotherms, species introduced from or to the Southern Hemisphere, and species introduced more recently. Model transferability was also positively associated with the number of presences for model calibration and evaluation, respectively, but negatively with the number of predictors. These findings highlight the importance of considering the characteristics of the focal invader, environment and modelling in the application and assessment of SDMs.View less
The Rum Layered Suite, NW Scotland, hosts Cr-spinel seams at the bases of peridotite-troctolite macro-rhythmic units in the eastern portion of the intrusion. Here, we present detailed field observations together with microstructural and mineral chemical analyses for the Unit 7-8 Cr-spinel seam and associated cumulates in the Eastern Layered Intrusion. Detailed mapping and sampling reveal significant lateral variations in the structural characteristics and mineral compositions of the Unit 7-8 boundary zone rocks. Although the Cr-spinel seam is laterally continuous over similar to 3 km, it is absent towards the centre and the margins of the intrusion. The compositional characteristics of Cr-spinel and plagioclase vary systematically along strike, exhibiting a chemical evolution towards more differentiated compositions with increasing distance from the main feeder conduit of the Rum intrusion; the Long Loch Fault. On the basis of our combined datasets, we propose that the upper part of the troctolite, the anorthosite layer underlying the Cr-spinel seam and the seam itself formed during a multi-stage magma replenishment event. The stages can be summarised as follows: (1) peridotite schlieren and anorthosite autoliths formed following melt infiltration and cumulate assimilation in the crystal mush of the Unit 7 troctolite. (2) The anorthosite layer then formed from the Unit 7 troctolite crystal mush by thermal erosion and dissolution due to infiltrating magma. (3) Subsequent dissolution of the anorthosite layer by new replenishing magma led to peritectic in situ crystallisation of the Unit 7-8 Cr-spinel seam, with (4) continued magma input eventually producing the overlying Unit 8 peridotite. In the central part of the Rum Layered Suite, the aforementioned assimilation of the troctolitic footwall formed the anorthosite layer. However, the absence of anorthosite in close proximity to the Long Loch Fault can be explained by enhanced thermochemical erosion close to the feeder zone, and its absence close to the margins of the intrusion, at maximum distance from the Long Loch Fault, may be due to cooling of the magma and loss of erosion potential. In line with other recent studies on PGE-bearing chromitites in layered intrusions, we highlight the importance of multi-stage intrusive magma replenishment to the formation of spatially coupled anorthosite and Cr-spinel seams, as well as the lateral mineral chemical variations observed in the Unit 7-8 boundary zone cumulates.View less
Objective: Obsessive-compulsive disorder (OCD) is a complex psychiatric disorder with a substantial genetic contribution. While the specific variants underlying OCD's heritability are still unknown, findings from genome-wide association studies (GWAS) corroborate the importance of common SNPs explaining the phenotypic variance in OCD. Investigating associations between the genetic liability for OCD, as reflected by a polygenic risk score (PRS), and potential endophenotypes of the disorder, such as the personality trait harm avoidance, may aid the understanding of functional pathways from genes to diagnostic phenotypes. Methods: We derived PRS for OCD at severalP-value thresholds based on the latest Psychiatric Genomics Consortium OCD GWAS (2688 cases, 7037 controls) in an independent sample of OCD patients (n = 180), their unaffected first-degree relatives (n = 108) and healthy controls (n = 200). Using linear regression, we tested whether these PRS are associated with the personality trait harm avoidance. Results: Results showed that OCD PRS significantly predicted OCD status, with patients having the highest scores and relatives having intermediate scores. Furthermore, the genetic risk for OCD was associated with harm avoidance across the entire sample, and among OCD patients. As indicated by mediation analyses, harm avoidance mediated the association between the OCD PRS and OCD caseness. These results were observed at multipleP-value thresholds and persisted after the exclusion of patients with a current comorbid major depressive or anxiety disorder. Conclusion: Our findings support the polygenic nature of OCD and further validate harm avoidance as a candidate endophenotype and diathesis of OCD.View less
The aim of this study is to develop nanometer-thin epoxy-based films on aluminium alloy AA2024-T3 as a model coating system for high resolution corrosion studies. Spin coating was used for the layer-by-layer (LbL) deposition of poly-(ethylenimine) (PEI) and poly([o-cresyl glycidyl ether]-co-formaldehyde) (CNER) bilayers. The film chemistry and the cross-linking process were characterized by means of Fourier-transform infrared spectroscopy (FTIR). Ellipsometric data confirmed the linear increase of film thickness. The potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) results indicate the improvement of the film barrier properties with increasing film thickness. Mapping of the topography and the volta potential was performed by means of scanning Kelvin probe force microscopy (SKPFM). The results indicate the presence of a homogeneous film structure, while the intermetallic phases can still be identified below the coating. The SKPFM analysis confirmed that the model films are suitable for investigation of corrosion processes at the coating/metal interface.View less
Isolation of biomolecules in vacuum facilitates characterization of the intramolecular interactions that determine three-dimensional structure, but experimental quantification of conformer thermochemistry remains challenging. Infrared spectroscopy of molecules trapped in helium nanodroplets is a promising methodology for the measurement of thermochemical parameters. When molecules are captured in a helium nanodroplet, the rate of cooling to an equilibrium temperature ofca.0.4 K is generally faster than the rate of isomerization, resulting in "shock-freezing" that kinetically traps molecules in local conformational minima. This unique property enables the study of temperature-dependent conformational equilibriaviainfrared spectroscopy at 0.4 K, thereby avoiding the deleterious effects of spectral broadening at higher temperatures. Herein, we demonstrate the first application of this approach to ionic species by coupling electrospray ionization mass spectrometry (ESI-MS) with helium nanodroplet infrared action spectroscopy to probe the structure and thermochemistry of deprotonated DNA dinucleotides. Dinucleotide anions were generated by ESI, confined in an ion trap at temperatures between 90 and 350 K, and entrained in traversing helium nanodroplets. The infrared action spectra of the entrained ions show a strong dependence on pre-pickup ion temperature, consistent with the preservation of conformer population upon cooling to 0.4 K. Non-negative matrix factorization was utilized to identify component conformer infrared spectra and determine temperature-dependent conformer populations. Relative enthalpies and entropies of conformers were subsequently obtained from a van't Hoff analysis. IR spectra and conformer thermochemistry are compared to results from ion mobility spectrometry (IMS) and electronic structure methods. The implementation of ESI-MS as a source of dopant molecules expands the diversity of molecules accessible for thermochemical measurements, enabling the study of larger, non-volatile species.View less
Inhibitory control, the inhibition of prepotent actions, is essential for higher-order cognitive processes such as planning, reasoning, and self-regulation. Individuals and species differ in inhibitory control. Identifying what influences inhibitory control ability within and between species is key to understanding how it evolved. We compared performance in the cylinder task across five lizard species: tree skinks (Egernia striolata), gidgee skinks (Egernia stokesii), eastern blue-tongue skinks (Tiliqua s. scincoides), sleepy lizards (Tiliqua r. asper), and eastern water skinks (Eulamprus quoyii). In our task, animals had to inhibit the prepotent motor response of directly approaching a reward placed within a semi-transparent mesh cylinder and instead reach in through the side openings. Additionally, in three lizard species, we compared performance in the cylinder task to reversal learning to determine the task specificity of inhibitory ability. Within species, neither sex, origin, body condition, neophobia, nor pre-experience with other cognitive tests affected individual performance. Species differed in motor response inhibition: Blue-tongue skinks made fewer contacts with the semi-transparent cylinder wall than all other species. Blue-tongue skinks also had lower body condition than the other species which suggest motivation as the underlying cause for species differences in task performance. Moreover, we found no correlation between inhibitory ability across different experiments. This is the first study comparing cylinder task performance among lizard species. Given that inhibitory control is probably widespread in lizards, motor response inhibition as exercised in the cylinder task appears to have a long evolutionary history and is likely fundamental to survival and fitness. Significance The study of lizard cognition is receiving increasing attention. Lizards are a diverse group with a wide range of ecological attributes and represent a model system through which we can test a wide range of hypotheses relating to cognitive evolution. Furthermore, considering their evolutionary history, studying non-avian reptile cognition can help understand the evolution of different cognitive abilities including inhibitory control. Here, we provide a comparison of inhibitory control ability in five lizard species. Consequently, we are able to, firstly, validate a method (the cylinder task) initially developed for the use in mammals and birds, for use in lizards, and secondly, collect valuable data on inhibitory control in a poorly studied group with respect to cognitive ability. Our study suggests non-cognitive factors as a major influence on cylinder task performance, which is in agreement with previous studies of other vertebrates.View less
Background Blood profile testing is commonly used to monitor herd health status, diagnose disorders, and predict the risk of diseases in cows and calves, with subsequent optimization the production of dairy herds. By understanding the physiological ranges of serum metabolites relative to age, lactation stage, and the sampling time in healthy cows and calves, the dairy practitioners can accurately diagnose abnormalities with a blood test. The effect of sampling time on the variation of serum metabolites within 24 h were evaluated in 83 cattle. All animals were originated from a dairy herd, where the animals, based on their ages and lactation stages, were classified into eight groups. The blood samples were collected from each animal every 4 h within a day. Results The time of sampling within the day showed significant influences on the serum concentrations of glucose, beta-hydroxybutyric acid (BHBA) and urea. BHBA was the most metabolite that showed day variation among cows' groups. Furthermore, the concentrations of total cholesterol were the most stable metabolite in all groups. The mean values of albumin, total proteins, glucose, non-esterified fatty acids (NEFA), BHBA, total cholesterol, total bilirubin, urea, and creatinine revealed significant variations among the different studied groups. Conclusions A certain suitable time of blood sample collection cannot be recommended. However, care shall be taken for the time of sampling for measurements of glucose, NEFA, BHBA and urea, otherwise the comparative values of these metabolites at different sampling time points may differ significantly from each other's, without a disease cause. It may be recommended, for metabolic assessment of dairy herds, classification the subjects into different groups based on lactation stages and ages of animals.View less
Within the framework of quantum refereed steering games, quantum steerability can be certified without any assumption on the underlying state nor the measurements involved. Such a scheme is termed the measurement-device-independent (MDI) scenario. Here, we introduce a measure of steerability in an MDI scenario, i.e., the result merely depends on the observed statistics and the quantum inputs. We prove that such a measure satisfies the convex steering monotone. Moreover, it is robust against not only measurement biases but also losses. We also experimentally estimate the amount of the measure with an entangled photon source. As two by-products, our experimental results provide lower bounds on an entanglement measure of the underlying state and an incompatible measure of the involved measurement. Our research paves a way for exploring one-side device-independent quantum information processing within an MDI framework.View less
Magnetic impurities on the surface of Rashba spin-orbit-coupled, but otherwise conventional, superconductors provide a promising way to engineer topological superconductors with Majorana bound states as boundary modes. In this work we show that the spin-polarization in the interior of both one-dimensional impurity chains and two-dimensional islands in these systems can be used to determine the superconducting topological phase, as it changes sign exactly at the topological phase transition. Thus, spin polarization offers an alternative method to detect the topological phase in magnetic impurity chains and islands deposited on conventional superconductors, beyond the zero-energy Majorana bound states.View less
Across group-living animals, linear dominance hierarchies lead to disparities in access to resources, health outcomes and reproductive performance. Studies of how dominance rank predicts these traits typically employ one of several dominance rank metrics without examining the assumptions each metric makes about its underlying competitive processes. Here, we compare the ability of two dominance rank metrics-simple ordinal rank and proportional or 'standardized' rank-to predict 20 traits in a wild baboon population in Amboseli, Kenya. We propose that simple ordinal rank best predicts traits when competition is density-dependent, whereas proportional rank best predicts traits when competition is density-independent. We found that for 75% of traits (15/20), one rank metric performed better than the other. Strikingly, all male traits were best predicted by simple ordinal rank, whereas female traits were evenly split between proportional and simple ordinal rank. Hence, male and female traits are shaped by different competitive processes: males are largely driven by density-dependent resource access (e.g. access to oestrous females), whereas females are shaped by both density-independent (e.g. distributed food resources) and density-dependent resource access. This method of comparing how different rank metrics predict traits can be used to distinguish between different competitive processes operating in animal societies.View less
We analyse the properties of an unbiased gradient estimator of the evidence lower bound (ELBO) for variational inference, based on the score function method with leave-one-out control variates. We show that this gradient estimator can be obtained using a new loss, defined as the variance of the log-ratio between the exact posterior and the variational approximation, which we call the log-variance loss. Under certain conditions, the gradient of the log-variance loss equals the gradient of the (negative) ELBO. We show theoretically that this gradient estimator, which we call VarGrad due to its connection to the log-variance loss, exhibits lower variance than the score function method in certain settings, and that the leave-one-out control variate coefficients are close to the optimal ones. We empirically demonstrate that VarGrad offers a favourable variance versus computation trade-off compared to other state-of-the-art estimators on a discrete variational autoencoder (VAE)View less
Background Effective and sustainable worm control in horses would benefit from detailed information about the current regional occurrence of tapeworms. Different diagnostic methods are currently available to detect Anoplocephala spp. infections in horses. However, the format as well as the sensitivity and specificity of the methods vary considerably.
Methods A coprological, serological and questionnaire study was conducted to investigate the prevalence and risk factors of tapeworm infections on 48 horse farms in the region of Berlin and Brandenburg, Germany. In total, faecal samples of 484 horses were analysed using the double centrifugation/combined sedimentation-flotation and mini-FLOTAC. Serum (n = 481) and saliva (n = 365) samples were analysed by ELISAs to determine antibody levels against Anoplocephala spp. 12/13 kDa excretory/secretory (E/S) antigens.
Results Cestode eggs were detected in 0.6% of faecal samples (farm prevalence 6.3%) without differences between the two methods. In contrast, antibodies against Anoplocephala spp. were detected in 16.2% (farm prevalence 52.1%) and in 29.5% (farm prevalence 75.7%) of the serum and saliva samples, respectively. Both ELISA based methods for detection of tapeworms reported a greater number of infected animals requiring treatment than were positively identified by coproscopy. Logistic regression analysis identified permanent pasture access, large pastures and regular pasture changes and high strongyle egg counts as risk factors for positive serum antibody responses to Anoplocephala spp. while last treatment with praziquantel was protective. Other protective factors were the presence of foals and high numbers of horses on the farm. Daily removal of faeces from the pasture and horse age did not have a significant effect.
Conclusions The findings of the present serological investigation indicate that tapeworm prevalence in Berlin/Brandenburg horse farms is much higher than would be anticipated by using conventional/coproscopic analyses. Moreover, the majority of tapeworm-positive horses had not received a cestocidal drug at their last treatment. Considering the already known low sensitivity of the coproscopic detection, the equine veterinary diagnostics can be enhanced by the use of antibody detection methods such as the saliva-based ELISA.View less
The shift from foraging to agriculture as an economic way of life can be influenced by multiple ecological and cultural factors. The introduction of rice cultivation in Japan appears to have facilitated a dietary and cultural transition from the Jomon to the Yayoi cultural repertoire (10th/4th century BCE). Here we examine how rice spread across the Yayoi cultural arena (Kyushu, Shikoku, and Honshu regions) using Bayesian modelling applied to a set of radiocarbon (C-14) dates obtained from carbonized rice grains. The combined results of radiocarbon analysis and archaeological data suggest that rice could have appeared in the Central Highlands already in the 11th century BCE when the region was occupied by people of the Final Jomon culture group and was mainly used for ritual purposes. It then appeared in western Japan (northern Kyushu) in the 9th century BCE and continued to disperse discontinuously across eastern Japan. This dispersal pattern likely results from the fusion of Jomon hunter -fisher-gatherer groups in eastern Japan with cultural traits introduced from the Eurasian mainland. The main driving factors for the immigration of early rice farmers into Japan (starting around 1000 BCE) appears to have been sociopolitical. Transformations in China led to the dissemination of rice farmers into the Korean Peninsula about 500 years earlier. The main drivers likely comprised: (i) the eastward expansion of the Shang dynasty (ca. 1600-1400 BCE); (ii) the eastward expansion of the Zhou kingdom, accompanied by the establishment of satellite states, such as Lu (Shandong Province) and Yan (Beijing), following the defeat of the Shang in 1045 BCE; and (iii) the strengthening of local states during the early 8th century BCE after the weakening of the Zhou, due to conflicts with agropastoralists from the Asian steppes. In addition, it is likely that the gradual middle-late Holocene decrease in summer monsoon precipitation negatively affected agricultural yields in the regions located closer to the summer monsoon boundary, such as the middle Yellow River, and thus further fostered the observed population dynamics including the spread of rice farmers to the Korean Peninsula and Japan.View less
In recent decades, different social innovations – such as lifelong learning, inclusion or Education for Sustainable Development – have had a huge impact on domestic education systems. In an increasingly globalized world, innovations diffuse across national borders. At the same time, diffusion processes seem to be highly influenced by public and private actors, e.g., international organizations (IOs) or non-governmental organizations (NGOs). Both state and non-state actors use social networks and digital communication platforms, such as Twitter, as channels for the diffusion of social innovations and practices. Inclusive education, which has become the main alternative to special schools for the schooling of children with disabilities, is a widely discussed innovation in education and, hence, represents a suitable case for the study of global diffusion processes and the involved actors. Thus, drawing on social network theory (SNT), the aim of this paper is to examine the structure of the Twitter communication network forming around the social innovation of inclusive education. Empirically, we use social network analysis (SNA) to map the communication network; identify central actors; and infer assumptions about the role of different actor groups. Our results show, for instance, that especially NGOs and IOs hold central positions in the network, which enables them to exert influence on the diffusion of innovative ideas. Overall, the findings of our study indicate how the online communication tool Twitter can play a crucial role for actors who seek to influence the global diffusion of social innovations in education and effect education policies, norms and systems at the global, national and regional levels as such.View less
Site-directed spin labeling (SDSL) of large RNAs for electron paramagnetic resonance (EPR) spectroscopy has remained challenging to date. We here demonstrate an efficient and generally applicable posttranscriptional SDSL method for large RNAs using an expanded genetic alphabet containing the NaM-TPT3 unnatural base pair (UBP). An alkyne-modified TPT3 ribonucleotide triphosphate (rTPT3(CO)TP) is synthesized and site-specifically incorporated into large RNAs byin vitrotranscription, which allows attachment of the azide-containing nitroxide through click chemistry. We validate this strategy by SDSL of a 419-nucleotide ribonuclease P (RNase P) RNA fromBacillus stearothermophilusunder non-denaturing conditions. The effects of site-directed UBP incorporation and subsequent spin labeling on the global structure and function of RNase P are marginal as evaluated by Circular Dichroism spectroscopy, Small Angle X-ray Scattering, Sedimentation Velocity Analytical Ultracentrifugation and enzymatic assay. Continuous-Wave EPR analyses reveal that the labeling reaction is efficient and specific, and Pulsed Electron-Electron Double Resonance measurements yield an inter-spin distance distribution that agrees with the crystal structure. The labeling strategy as presented overcomes the size constraint of RNA labeling, opening new avenues of spin labeling and EPR spectroscopy for investigating the structure and dynamics of large RNAs.View less
Using pulsed electron paramagnetic resonance (EPR) techniques, the low-temperature magnetic properties of the NO radical being confined in two different modified open C60-derived cages are determined. It is found that the smallest principal g value g3, being assigned to the axis of the radical, deviates strongly from the free electron value. This behaviour results from partial compensation of the spin and orbital contributions to the g3 value. The measured g3 values in the range of 0.7 yield information about the deviation of the locking potential for the encaged NO from axial symmetry. The estimated 17 meV asymmetry is quite small compared to the situation found for the same radical in polycrystalline or amorphous matrices ranging from 300 to 500 meV. The analysis of the temperature dependence of spin relaxation times resulted in an activation temperature of about 3 K, assigned to temperature-activated motion of the NO within the modified open C60-derived cages with coupled rotational and translational degrees of freedom in a complicated three-dimensional locking potential.View less
We describe a new data structure for dynamic nearest neighbor queries in the plane with respect to a general family of distance functions. These include L-p-norms and additively weighted Euclidean distances. Our data structure supports general (convex, pairwise disjoint) sites that have constant description complexity (e.g., points, line segments, disks, etc.). Our structure uses O(n log(3)n) storage, and requires polylogarithmic update and query time, improving an earlier data structure of Agarwal, Efrat, and Sharir which required O(n(epsilon)) time for an update and O(log n) time for a query [SICOMP 1999]. Our data structure has numerous applications. In all of them, it gives faster algorithms, typically reducing an O(n(epsilon)) factor in the previous bounds to polylogarithmic. In addition, we give here two new applications: an efficient construction of a spanner in a disk intersection graph, and a data structure for efficient connectivity queries in a dynamic disk graph. To obtain this data structure, we combine and extend various techniques from the literature. Along the way, we obtain several side results that are of independent interest. Our data structure depends on the existence and an efficient construction of "vertical" shallow cuttings in arrangements of bivariate algebraic functions. We prove that an appropriate level in an arrangement of a random sample of a suitable size provides such a cutting. To compute it efficiently, we develop a randomized incremental construction algorithm for computing the lowest k levels in an arrangement of bivariate algebraic functions (we mostly consider here collections of functions whose lower envelope has linear complexity, as is the case in the dynamic nearest-neighbor context, under both types of norm). To analyze this algorithm, we also improve a longstanding bound on the combinatorial complexity of the vertical decomposition of these levels. Finally, to obtain our structure, we combine our vertical shallow cutting construction with Chan's algorithm for efficiently maintaining the lower envelope of a dynamic set of planes in R-3. Along the way, we also revisit Chan's technique and present a variant that uses a single binary counter, with a simpler analysis and improved amortized deletion time (by a logarithmic factor; the insertion and query costs remain asymptotically the same).View less
We present a nanospectroscopic device platform allowing simple and spatially resolved thermoelectric detection of molecular fingerprints of soft materials. Our technique makes use of a locally generated thermal gradient converted into a thermoelectric photocurrent that is read out in the underlying device. The thermal gradient is generated by an illuminated atomic force microscope tip that localizes power absorption onto the sample surface. The detection principle is illustrated using a concept device that contains a nanostructured strip of polymethyl methacrylate (PMMA) defined by electron beam lithography. The platform's capabilities are demonstrated through a comparison between the spectrum obtained by on-chip thermoelectric nanospectroscopy with a nano-FTIR spectrum recorded by scattering-type scanning near-field optical microscopy at the same position. The subwavelength spatial resolution is demonstrated by a spectral line scan across the edge of the PMMA layer.View less