As part of recent years’ efforts at reaching a more context- and diversity-sensitive study of international relations, the nexus between fields of IR and Area Studies (AS) has received a renewed attention. While AS is usually presented as the “contextualizer” of the disciplines, this forum reverses the perspective by suggesting that an awareness of both diversity and context is also relevant when it comes to understanding the evolution of the field of AS and its relations to IR. In this forum, a selection of scholars with diverse backgrounds (US, Middle East, Europe, Latin America, Africa, and Central Asia), different (inter)disciplinary trainings and regional orientations examines how various fields of AS and its relations to the disciplines vary, and what follows from a stronger attention to such kind of diversity. By contextualizing the contextualizers, the forum brings attention to how a context-sensitive field can also suffer from its own provincialism. While the US-centric narrative about AS might have been almost “hegemonic,” at closer inspection, it turns out that AS in different (sub)disciplinary and geographical settings have evolved differently, and in some places the so-called Area Studies controversy (ASC) has been almost absent. A broadening of the perspective also reveals how the challenges to a successful cross-fertilization are not limited to those outlined in the “classic” ASC, but the forum does simultaneously offer encouraging lessons on how dialogues between area specialists and discipline-oriented scholars can help to overcome epistemological, theoretical, or methodological blind spots. Rather than presenting the IR/AS nexus as a panacea per se, the aim of the forum is therefore to invite to a broader and more self-reflective discussion on some of the opportunities as well as challenges associated with this strategy for making the study of international relations more context-sensitive and attentive to different forms of diversity.
Weniger anzeigenBackground
The United Arab Emirates (UAE) has witnessed rapid urbanization and a surge in pet ownership, sparking concerns about the possible transfer of antimicrobial resistance (AMR) from pets to humans and the environment. This study delves into the whole-genome sequencing analysis of ESBL-producing E. coli strains from healthy cats and dogs in the UAE, which exhibit multidrug resistance (MDR). Additionally, it provides a genomic exploration of the mobile colistin resistance gene mcr-1.1, marking the first instance of its detection in Middle Eastern pets.
Methods
We investigate 17 ESBL-producing E. coli strains from healthy UAE pets using WGS and bioinformatics analysis to identify genes encoding virulence factors, assign diverse typing schemes to the isolates, and scrutinize the presence of AMR genes. Furthermore, we characterized plasmid contigs housing the mcr-1.1 gene and conducted phylogenomic analysis to evaluate their relatedness to previously identified UAE isolates.
Results
Our study unveiled a variety of virulence factor-encoding genes within the isolates, with fimH emerging as the most prevalent. Regarding β-lactamase resistance genes, the blaCTX group 1 gene family predominated, with CTX-M-15 found in 52.9% (9/17) of the isolates, followed by CTX-M-55 in 29.4% (5/17). These isolates were categorized into multiple sequence types (STs), with the epidemic ST131 being the most frequent. The presence of the mcr-1.1 gene, linked to colistin resistance, was confirmed in two isolates. These isolates belonged to ST1011 and displayed distinct profiles of β-lactamase resistance genes. Phylogenomic analysis revealed close connections between the isolates and those from chicken meat in the UAE.
Conclusion
Our study underscores the presence of MDR ESBL-producing E. coli in UAE pets. The identification of mcr-1.1-carrying isolates warrants the urgency of comprehensive AMR surveillance and highlights the role of companion animals in AMR epidemiology. These findings underscore the significance of adopting a One Health approach to mitigate AMR transmission risks effectively.
Weniger anzeigenSkyrmions are localized, topological spin structures that can be described as quasiparticles. Skyrmions in thin films are an ideal model system to study Brownian motion and lattice formation in two dimensions. They follow an equation of motion, the Thiele equation, which includes a topology-dependent chiral term, linear in velocity, causing a skyrmion Hall effect and a drastic reduction of the diffusion coefficient for individual skyrmions, as compared to normal Brownian particles. Using Brownian dynamics simulations, we show that this topological suppression of the diffusion can be partially lifted in two-dimensional lattices of skyrmions. Counterintuitively, this causes enhanced diffusive properties with increasing particle density, similar to odd-diffusive Brownian particles. We show how the topological charge of the skyrmions influences the dynamics of topological lattice defects, which also affects the dynamics of the phase formation.
Weniger anzeigenCopper isotopes (δ65Cu) in hydrothermal fluids have the potential to provide information on ore-forming processes occurring below the seafloor, but Cu isotope data from high-temperature fluids are scarce. Here, we examine the extent to which coexisting sulfide minerals in a hydrothermal chimney can preserve fluid Cu isotope ratios using a fluid-solid pair of a black smoker (333°C) from the Roman Ruins vent area (PACMANUS) in the Manus Basin. Two ca. 3 cm long transects through the chalcopyrite-rich chimney wall show an increase in δ65Cu from 0.48 to 2.28‰ from the interior to the exterior, coupled with limited variation in sulfide δ34S (1.52–4.72‰). The Cu isotopic composition of chalcopyrite from the innermost wall closely resembles the δ65Cu value of the paired hydrothermal fluid, indicating that chalcopyrite in the inner ∼5 mm of the chimney records the Cu isotope ratio of the venting fluid. Beyond this, an increase in sulfide δ65Cu toward the exterior correlates with an increase in the relative abundance of secondary Cu sulfides. The appearance of bornite coincides with the presence of small barite crystals, suggesting this represents a redox gradient between reduced hydrothermal fluids and oxidized seawater admixing inwards. Elevated δ65Cu in this zone can be explained by the precipitation of secondary Cu sulfides from 65Cu-enriched fluids formed during oxidative chalcopyrite dissolution. Our findings indicate that interactions with oxidizing seawater shift chalcopyrite δ65Cu values over small spatial scales, and that caution must be applied if chimney sulfides are used to reconstruct δ65Cu values of high-temperature hydrothermal fluids.
Weniger anzeigenFlyovers have featured in critical urban planning scholarship in the Global South as fetishized symbols of modernity, often fragmenting urban environments, fracturing space, exacerbating inequalities, and embodying “worlding”’ aspirations of city planners. Acknowledging the role of such infrastructure as technologies of (dis)connection in increasingly enclaved cities, I seek to situate the flyover, its material form, and attendant gaps, characterized by raised ribbons of “smooth” flows, leftover spaces, and proliferation of informal practices, as important sites of encounters. As such, I take “borderland urbanism” as an impetus to think flyover geographies anew by locating the flyover as a particular place in the city that is transient, contested, and constantly re-made. Through ethnographic vignettes and interviews, I sketch out everyday urban experiences over and under a flyover in Karachi, Pakistan. I illustrate how the flyover as a spatial and temporal leap is perceived and experienced by a range of differently mobile urban dwellers, paying particular attention to how walking bodies inhabit an infrastructural landscape that heavily privileges cars and motorcycles. Furthermore, I trace how life in the interstices under the flyover is assembled through social collaboration, resisting eviction, and a politics of visibility.
Weniger anzeigenThis work analyses the symbols and utopian thought in the book of Ibn Bājja as well as his philosophy about the purpose of the human being to reach perfection and the essence of life. Through reading closely his work Tadbīr al-mutawaḥḥid and with some other sources about the work, I formulated some questions which I will then answer in this essay. From Ibn Bājja’s, what are the ways to achieve perfection ? How do solitary people achieve a utopian life? What is the meaning of al-mutawaḥḥid (solitary)? Tadbīr al-mutawaḥḥid was used for analysis to answer these questions. By reading this book, I can directly understand Ibn Bajja's philosophy on utopia and the idea of solitude from the corrupt society and upgrading the soul with virtuous morals. Also there are secondary sources, between articles and books that analyse Ibn Bājja’s book, as well as discuss the idea of isolation from society. In order to be able at the end of this work to conclude whether the Ibn Bājja’s work leads a utopian life or not, it was necessary to define the concept of utopianism. There are some general sources that give a brief overview of what Utopia is, as well as compar it with the utopianism of Ibn Bājja in his book Tadbīr al-mutawaḥḥid. It'll be interesting at the start of this essay to introduc the author of the book Tadbīr al-mutawaḥḥid. This summary introductory of the aspects and circumstances of his life may be of some help in understanding the idea of his book, especially the idea of isolation and loneliness.
Weniger anzeigenHaving a tool to monitor the microbial abundances rapidly and to utilize the data to predict the reactor performance would facilitate the operation of an anaerobic membrane bioreactor (AnMBR). This study aims to achieve the aforementioned scenario by developing a linear regression model that incorporates a time-lagging mode. The model uses low nucleic acid (LNA) cell numbers and the ratio of high nucleic acid (HNA) to LNA cells as an input data set. First, the model was trained using data sets obtained from a 35 L pilot-scale AnMBR. The model was able to predict the chemical oxygen demand (COD) removal efficiency and methane production 3.5 days in advance. Subsequent validation of the model using flow cytometry (FCM)-derived data (at time t – 3.5 days) obtained from another biologically independent reactor did not exhibit any substantial difference between predicted and actual measurements of reactor performance at time t. Further cell sorting, 16S rRNA gene sequencing, and correlation analysis partly attributed this accurate prediction to HNA genera (e.g., Anaerovibrio and unclassified Bacteroidales) and LNA genera (e.g., Achromobacter, Ochrobactrum, and unclassified Anaerolineae). In summary, our findings suggest that HNA and LNA cell routine enumeration, along with the trained model, can derive a fast approach to predict the AnMBR performance.
Weniger anzeigenIn this article, we study the quantum field theoretic generalization of the Caldeira-Leggett model in general curved space-time considering interactions between two scalar fields in a classical gravitational background. The thermalization phenomena is then studied from the obtained de Sitter solution using quantum quench from one scalar field model obtained from path integrated effective action. We consider an instantaneous quench in the time-dependent mass protocol of the field of our interest. We find that the dynamics of the field post-quench can be described in terms of the state of the generalized Calabrese-Cardy (gCC) form and computed the different types of two-point correlation functions in this context. We explicitly found the conserved charges of algebra that represents the gCC state after a quench in de Sitter space and found it to be significantly different from the flat space-time results. We extend our study for the different two-point correlation functions not only considering the pre-quench state as the ground state, but also a squeezed state. We found that irrespective of the pre-quench state, the post quench state can be written in terms of the gCC state showing that the subsystem of our interest thermalizes in de Sitter space. Furthermore, we provide a general expression for the two-point correlators and explicitly show the thermalization process by considering a thermal Generalized Gibbs ensemble (GGE). Finally, from the equal time momentum dependent counterpart of the obtained results for the two-point correlators, we have studied the hidden features of the power spectra and studied its consequences for different choices of the quantum initial conditions.
Weniger anzeigenWhile nighttime curfews are less severe restrictions compared to around-the-clock curfews in mitigating the spread of Covid-19, they are nevertheless highly controversial, with the scarce literature on their effectiveness providing mixed evidence. We study the effectiveness of the nighttime curfew in Hamburg, Germany’s second largest city, in mitigating the spread of Covid-19. This curfew forbid people from leaving their home between 9 p.m. and 5 a.m. for non-essential businesses. Applying both difference-in-differences and synthetic control group methods, we find that the curfew was effective in reducing the number of Covid-19 cases. As it is unclear whether and how the virus will mutate in the next time, policy-makers might have to resort to non-pharmaceutical interventions again. Nighttime curfews should be kept in the toolbox of policy-makers to fight Covid-19.
Weniger anzeigenFibroblast growth factor 2 (FGF2) exits cells by direct translocation across the plasma membrane, a type I pathway of unconventional protein secretion. This process is initiated by phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2)-dependent formation of highly dynamic FGF2 oligomers at the inner plasma membrane leaflet, inducing the formation of lipidic membrane pores. Cell surface heparan sulfate chains linked to glypican-1 (GPC1) capture FGF2 at the outer plasma membrane leaflet, completing FGF2 membrane translocation into the extracellular space. While the basic steps of this pathway are well understood, the molecular mechanism by which FGF2 oligomerizes on membrane surfaces remains unclear. In the current study, we demonstrate the initial step of this process to depend on C95-C95 disulfide-bridge-mediated FGF2 dimerization on membrane surfaces, producing the building blocks for higher FGF2 oligomers that drive the formation of membrane pores. We find FGF2 with a C95A substitution to be defective in oligomerization, pore formation, and membrane translocation. Consistently, we demonstrate a C95A variant of FGF2 to be characterized by a severe secretion phenotype. By contrast, while also important for efficient FGF2 secretion from cells, a second cysteine residue on the molecular surface of FGF2 (C77) is not involved in FGF2 oligomerization. Rather, we find C77 to be part of the interaction interface through which FGF2 binds to the α1 subunit of the Na,K-ATPase, the landing platform for FGF2 at the inner plasma membrane leaflet. Using cross-linking mass spectrometry, atomistic molecular dynamics simulations combined with a machine learning analysis and cryo-electron tomography, we propose a mechanism by which disulfide-bridged FGF2 dimers bind with high avidity to PI(4,5)P2 on membrane surfaces. We further propose a tight coupling between FGF2 secretion and the formation of ternary signaling complexes on cell surfaces, hypothesizing that C95-C95-bridged FGF2 dimers are functioning as the molecular units triggering autocrine and paracrine FGF2 signaling.
Weniger anzeigenBackground: During the COVID-19 pandemic, governments worldwide issued lockdown and social-distancing measures, which triggers psychological distress and may increase the occurrence of domestic violence (DV). We examined the role of the LGBT+ status in the relationship between risk factors of DV and its occurrence during the pandemic. Methods: In this cross-sectional study (n = 5,148), an online self-report questionnaire was administered to a non-probabilistic sample of participants living in Belgium, Germany, and Portugal between March and June 2020. Participants were sampled through national media, social media, and snowballing procedures. Results: LGBT+ persons reported significantly higher levels of perceived stress, more acute stress symptoms, and more illegal drug use during the first weeks of the lockdown compared to non-LGBT+ persons. DV in LGBT+ persons was not more prevalent than in the non-LGBT under COVID-19 lockdown measures. No significant evidence was found indicating that identifying as LGBT+ itself increases the risk of DV exposure. Conclusion: LGBT+ persons were more at risk of DV compared to non-LGBT+ persons prior to but not during COVID-19 lockdown measures. The increased risk for DV can be linked to socio-demographic characteristics, levels of stress, and illegal drug use which are more prevalent in LGBT+ persons than in non-LGBT+ persons.
Weniger anzeigenR&D capacity is claimed to be a stimulant for open innovation. We argue that after a specific level of R&D investment, firms diminish their external innovation collaboration due to the risk of knowledge imitation and unwanted spillovers, which generates a concave relationship between the two variables. This concave relationship can be flattened if an appropriation strategy is applied. Using 3815 firm observations from the German Mannheim Innovation Panel (MIP) in 2013 and 2017, our regression results support our hypotheses. Theoretically, our study suggests that the absorptive capacity literature should consider the potential negative impact of internal R&D capacity on openness. Similarly, economists should approach knowledge spillovers as a manageable issue if an appropriation strategy is present. Specifically, appropriation stimulates openness by playing a double role in protecting internal knowledge against imitation while also sending a quality signal for external partners to collaborate. This effect is driven by the presence of an appropriation strategy regardless of its form or strength.
Weniger anzeigenTo confront the climate crisis requires fundamental system change in order to break the convention of relentless economic exploitation of nature. In this Special Issue we extend understanding of the opportunities for an organizing perspective on sustainability in order that organization studies might contribute more effectively to the challenges of organizing sustainably. This organizing perspective is particularly sensitive to (1) a variety of forms and practices of sustainable organizing in different societal spheres and on different levels, (2) the social institutions, logics and value systems in which these forms and practices are embedded, (3) the power and politics of promoting (or blocking) sustainable organization, and (4) the ways in which work, voice, participation, and inclusion are organized and contribute to developing societal capabilities. These features formed the basis of our original call for papers and we review selected literature on sustainability, including the contribution of organization studies and the articles in this Special Issue, through this organizing perspective. In so doing we identify four key themes of a future research agenda that builds from the foundations of existing research and addresses key current limitations in both theory and practice: sustainability requires social justice; connecting local and global scale shifts; democratizing governance; and acting collectively. We conclude with some implications for our own scholarship in organization studies if we are to meet the twin challenges of the need for new theorizing in combination with devising practically relevant support for change.
Weniger anzeigenThe NASA MODIS MOD10A1 snow albedo product has enabled numerous glaciological applications. The temporal consistency of MODIS albedo is critical to obtaining reliable results from this 22-year time series. The orbit of Terra began to drift toward earlier acquisition times after the final inclination adjustment maneuver to maintain its nominal orbit by NASA on 27 February 2020, which may introduce biases that compromise the accuracy of quantitative time series analysis as the drift continues. Here, we evaluate the impact of Terra's orbital drift by comparing the differences between the Terra MODIS albedo and albedo products derived from Aqua MODIS, harmonized Landsat and Sentinel 2, Sentinel 3, and PROMICE (Programme for Monitoring of the Greenland Ice Sheet) ground measurements over the Greenland ice sheet. Our results suggest that the influence of orbital drift on albedo is small (+0.01 in 2020), but potentially biased for time series analysis. Our analysis also finds that the drift effect that causes earlier image acquisition time may lead to more apparently cloudy pixels and thus effectively reduce the Terra MODIS temporal resolution over Greenland.
Weniger anzeigenHfO2-based thin films hold huge promise for integrated devices as they show full compatibility with semiconductor technologies and robust ferroelectric properties at nanometer scale. While their polarization switching behavior has been widely investigated, their electromechanical response received much less attention so far. Here, we demonstrate that piezoelectricity in Hf0.5Zr0.5O2 ferroelectric capacitors is not an invariable property but, in fact, can be intrinsically changed by electrical field cycling. Hf0.5Zr0.5O2 capacitors subjected to ac cycling undergo a continuous transition from a positive effective piezoelectric coefficient d33 in the pristine state to a fully inverted negative d33 state, while, in parallel, the polarization monotonically increases. Not only can the sign of d33 be uniformly inverted in the whole capacitor volume, but also, with proper ac training, the net effective piezoresponse can be nullified while the polarization is kept fully switchable. Moreover, the local piezoresponse force microscopy signal also gradually goes through the zero value upon ac cycling. Density functional theory calculations suggest that the observed behavior is a result of a structural transformation from a weakly-developed polar orthorhombic phase towards a well-developed polar orthorhombic phase. The calculations also suggest the possible occurrence of a non-piezoelectric ferroelectric Hf0.5Zr0.5O2. Our experimental findings create an unprecedented potential for tuning the electromechanical functionality of ferroelectric HfO2-based devices.
Weniger anzeigenWetland restoration has received increasing attention to compensate for the continuing loss of natural wetlands and revive biodiversity and associated ecological functions. The development of methods for assessing the effectiveness of restoration measures is important for wetland management. However, most studies assessing restoration success in freshwater ecosystems are based on taxonomic biodiversity; trait-based approaches remain limited, particularly in wetland ecosystems. Here, we assess the effectiveness of wetland restoration in the Sanjiang Plain, Northeast China, based on the trait composition and functional diversity of aquatic insect assemblages. We found that functional richness in restored wetlands was significantly higher than impacted wetlands but lower than natural wetlands. Compared to natural wetlands, functional beta diversity of impacted wetlands was higher. In terms of dissimilarity between wetland types, trait composition of aquatic insects in restored wetlands showed a higher similarity to natural wetlands compared to those in impacted wetlands. Trait compositions of aquatic insect assemblages in restored wetlands were more similar to impacted wetlands in May but showed a greater similarity to natural wetlands in October. Our study shows that restoration measures have facilitated the partial recovery of functional diversity and trait composition of aquatic insect assemblages in the Sanjiang Plain wetlands. Our study confirms the potential of using trait-based approach to assess the effectiveness of wetland restoration. Given that assessments based on taxonomic and trait data can provide complementary information, we recommend incorporating both taxonomic and functional metrics and considering seasonal dynamics of wetlands in post-restoration monitoring and assessment in wetlands.
Weniger anzeigenPolycomb Repressive Complexes (PRCs) control gene expression through the incorporation of H2Aub and H3K27me3. In recent years, there is increasing evidence of the complexity of PRCs’ interaction networks and the interplay of these interactors with PRCs in epigenome reshaping, which is fundamental to understand gene regulatory mechanisms. Here, we identified UBIQUITIN SPECIFIC PROTEASE 5 (UBP5) as a chromatin player able to counteract the deposition of the two PRCs’ epigenetic hallmarks in Arabidopsis thaliana. We demonstrated that UBP5 is a plant developmental regulator based on functional analyses of ubp5-CRISPR Cas9 mutant plants. UBP5 promotes H2A monoubiquitination erasure, leading to transcriptional de-repression. Furthermore, preferential association of UBP5 at PRC2 recruiting motifs and local H3K27me3 gaining in ubp5 mutant plants suggest the existence of functional interplays between UBP5 and PRC2 in regulating epigenome dynamics. In summary, acting as an antagonist of the pivotal epigenetic repressive marks H2Aub and H3K27me3, UBP5 provides novel insights to disentangle the complex regulation of PRCs’ activities.
Weniger anzeigenAdaptive decision-making is governed by at least two types of memory processes. On the one hand, learned predictions through integrating multiple experiences, and on the other hand, one-shot episodic memories. These two processes interact, and predictions – particularly prediction errors – influence how episodic memories are encoded. However, studies using computational models disagree on the exact shape of this relationship, with some findings showing an effect of signed prediction errors and others showing an effect of unsigned prediction errors on episodic memory. We argue that the choice-confirmation bias, which reflects stronger learning from choice-confirming compared to disconfirming outcomes, could explain these seemingly diverging results. Our perspective implies that the influence of prediction errors on episodic encoding critically depends on whether people can freely choose between options (i.e., instrumental learning tasks) or not (Pavlovian learning tasks). The choice-confirmation bias on memory encoding might have evolved to prioritize memory representations that optimize reward-guided decision-making. We conclude by discussing open issues and implications for future studies.
Weniger anzeigenInfluenza A virus (IAV) defective interfering particles (DIPs) are considered as new promising antiviral agents. Conventional DIPs (cDIPs) contain a deletion in the genome and can only replicate upon co-infection with infectious standard virus (STV), during which they suppress STV replication. We previously discovered a new type of IAV DIP “OP7” that entails genomic point mutations and displays higher antiviral efficacy than cDIPs. To avoid safety concerns for the medical use of OP7 preparations, we developed a production system that does not depend on infectious IAV. We reconstituted a mixture of DIPs consisting of cDIPs and OP7 chimera DIPs, in which both harbor a deletion in their genome. To complement the defect, the deleted viral protein is expressed by the suspension cell line used for production in shake flasks. Here, DIP preparations harvested are not contaminated with infectious virions, and the fraction of OP7 chimera DIPs depended on the multiplicity of infection. Intranasal administration of OP7 chimera DIP material was well tolerated in mice. A rescue from an otherwise lethal IAV infection and no signs of disease upon OP7 chimera DIP co-infection demonstrated the remarkable antiviral efficacy. The clinical development of this new class of broad-spectrum antiviral may contribute to pandemic preparedness.
Weniger anzeigenSynthetic pesticides are widely applied in modern agriculture, where they are used against diseases, pests, and weeds to secure crop yield and quality. However, their intensive application has led to widespread contamination of the environment, including soils. Due to their inherent toxicity, they might pose a risk to soil health by causing harm to non-target organisms and disrupting ecosystem services in both agricultural and other exposed soils. Following the Swiss National Action Plan on the reduction of pesticide risks, Agroscope has conducted several soil monitoring studies that are briefly presented here. All of them resort to different multi-residue trace analytical approaches to simultaneously quantify up to about 150 modern pesticides by either accelerated solvent, or Quick, Easy, Cheap, Efficient, Rugged, Safe (QuEChERS) extraction, followed by separation and detection with liquid chromatography-triple quadrupole mass spectrometry. While partly still in progress, our investigations led to the following major findings this far: Multiple pesticides are commonly present in soils, with individual concentrations in agricultural soils often reaching up to a few tens of µg/kg. Pesticide occurrence and concentrations in agricultural soils primarily depend on land use, land use history and cultivated crops. Pesticides can prevail much longer than predicted by their half-lives, and were found in soils even decades after conversion from conventional to organic farming. Corresponding residual fractions can be in the order of a few percent of the originally applied amounts. We further found negative associations of pesticide residues with the abundance of beneficial soil life, underpinning their potential risk to the fertility of agricultural soils. Traces of pesticides are also detected in soils to which they were never applied, indicating contamination, e.g., via spray drift or atmospheric deposition. These results confirm the general notion of both scientists and legislators that prospective risk assessments (RA; as executed during registration and use authorization) should be confirmed and adjusted by retrospective RA (e.g., by environmental monitoring studies of currently used compounds) to jointly lead to an overall reduced environmental risk of pesticides.
Weniger anzeigen