Polo is an equestrian sport with a long tradition, but to date there is little scientific data on health risks, particularly lameness. In an international online survey, 145 polo players (47.6% female, 52.4% male) with an average of 13.4 years of playing experience were questioned about lameness risks and influencing factors. The respondents reported a median of six horses, most frequently mares as their best-performing and healthiest animals. Horse age was significantly associated with the risk of lameness, increasing by approximately 19.5% per year (OR = 1.195; p = 0.030) and differed depending on the breed of horse. Player-related variables such as age, sex, level of experience and type of husbandry had no significant influence. Risk perception was only associated with the handicap: players with a higher handicap rated the risk of injury as lower. Sex, age and horse ownership were not relevant. Unfavourable ground conditions were cited as the most common cause of injury (58.6%). The results show that players believe key predictors of lameness, in particular the age and breed of the horses, are objective factors, while subjective risk assessments are primarily influenced by the players’ level of experience. Further studies are necessary to improve horse health in polo and to enable preventive measures.