Proton-Coupled Electron Transfer in Cytochrome c Oxidase: Heme a Controls the Protonation Dynamics of E286

Abstract

Complex IV of the mitochondrial respiratory chain, or cytochrome c oxidase (CcO), contributes to the proton motive force necessary for ATP synthesis. CcO can slow the formation of reactive oxygen species and is key to physiology and drug development. The exact molecular mechanisms underlying its proton-pumping function remain elusive. The redox state of CcO's metallic cofactors is intimately connected to structural changes and proton pumping via proton-coupled electron transfer. Time-resolved UV/Vis and IR spectroscopy are used to investigate the effects of the electronic backreaction triggered by photolyzing the CO-inhibited 2-electron reduced state, R2CO, in the aa3 oxidase from Cereibacter sphaeroides. An intermediate is identified, in which the binuclear center matches the redox state of the catalytic intermediate E (one-electron reduced state), with a rise time of ≈2 μs. The electron transfer induces structural changes that lead to E286 deprotonation, with a time constant of 13 μs. Thus, it is inferred that transient reduction of heme a alone drives E286 deprotonation. E286 is reprotonated with a time constant of 72 ms when CO rebinds. The results support the view that transient heme a reduction in the physiological E state modulates the electrostatic environment, triggering proton transfer toward the proton-loading site.