dc.contributor.author
Huber, Lukas)
dc.contributor.author
Kuropka, Benno
dc.contributor.author
Doulidis, Pavlos G.
dc.contributor.author
Baszler, Elisabeth
dc.contributor.author
Martin, Lukas
dc.contributor.author
Rosu, Anda
dc.contributor.author
Kulmer, Lisa
dc.contributor.author
Ramos, Carolina Frizzo
dc.contributor.author
Rodríguez-Rojas, Alexandro
dc.contributor.author
Burgener, Iwan A.
dc.date.accessioned
2024-05-15T12:15:19Z
dc.date.available
2024-05-15T12:15:19Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/43567
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-43283
dc.description.abstract
Acute haemorrhagic diarrhoea is a common complaint in dogs. In addition to causes like intestinal parasites, dietary indiscretion, intestinal foreign bodies, canine parvovirus infection, or hypoadrenocorticism, acute haemorrhagic diarrhoea syndrome (AHDS) is an important and sometimes life-threatening differential diagnosis. There is some evidence supporting the link between Clostridium perfringens toxins and AHDS. These toxins may be partially responsible for the epithelial cell injury, but the pathogenesis of AHDS is still not fully understood. Recent studies have suggested that severe damage to the intestinal mucosa and associated barrier dysfunction can trigger chronic gastrointestinal illnesses. Besides bloodwork and classical markers for AHDS such as protein loss and intestinal bacterial dysbiosis, we focused mainly on the plasma-proteome to identify systemic pathological alterations during this disease and searched for potential biomarkers to improve the diagnosis. To accomplish the goals, we used liquid chromatography-mass spectrometry. We compared the proteomic profiles of 20 dogs with AHDS to 20 age-, breed-, and sex-matched control dogs. All dogs were examined, and several blood work parameters were determined and compared, including plasma biochemistry and cell counts. We identified and quantified (relative quantification) 207 plasmatic proteins, from which dozens showed significantly altered levels in AHDS. Serpina3, Lipopolysaccharide-binding protein, several Ig-like domain-containing proteins, Glyceraldehyde-3-phosphate dehydrogenase and Serum amyloid A were more abundant in plasma from AHDS affected dogs. In contrast, other proteins such as Paraoxonase, Selenoprotein, Amine oxidases, and Apolipoprotein C-IV were significantly less abundant. Many of the identified and quantified proteins are known to be associated with inflammation. Other proteins like Serpina3 and RPLP1 have a relevant role in oncogenesis. Some proteins and their roles have not yet been described in dogs with diarrhoea. Our study opens new avenues that could contribute to the understanding of the aetiology and pathophysiology of AHDS.
en
dc.format.extent
16 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Blood plasma
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Plasma proteome signature of canine acute haemorrhagic diarrhoea syndrome (AHDS)
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e0297924
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0297924
dcterms.bibliographicCitation.journaltitle
PLoS ONE
dcterms.bibliographicCitation.number
2
dcterms.bibliographicCitation.volume
19
dcterms.bibliographicCitation.url
https://doi.org/10.1371/journal.pone.0297924
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1932-6203
refubium.resourceType.provider
WoS-Alert