The Tieshajie Cu deposit, located in the northeastern part of the Qin-Hang Metallogenic Belt (QHMB), South China, has long been regarded as a representative Meso-Neoproterozoic volcanogenic massive sulfide (VMS) deposit. Here we present a hydrothermal titanite U-Pb age, Re-Os and in-situ S-Cu isotope data for chalcopyrite to constrain the timing and ore genesis of the Tieshajie deposit. Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) U-Pb dating of titanite from the disseminated Cu ore yielded a weighted mean 206Pb/238U age of 160.1 ± 4.4 Ma. Chalcopyrite from different ore types has low 187Os/188Os (0.85–3.60) and 187Re/188Os (46.1–614.0) ratios, combined with initial 187Os/188Os (0.74–2.00), excluding a mantle source. A Re-Os isochron age (188 ± 30 Ma) for five chalcopyrite samples is consistent with the titanite U-Pb age within errors. Moreover, the variations in Cu isotope compositions (δ65Cu: −1.13 to +0.12 ‰) and δ34S values (+3.8 to +7.7 ‰) of chalcopyrite are inconsistent with those reported from the ancient VMS deposits in previous studies. Therefore, our results are indicative of a Late Jurassic magmatic-hydrothermal origin instead of a VMS origin for the Tieshajie deposit. In combination with previous studies, we propose that the Tieshajie Cu deposit belongs to the distal part of the Mid-Late Jurassic (170–150 Ma) porphyry-skarn Cu mineralization event in the QHMB, likely triggered by the subduction of the Paleo-Pacific plate during the Late Mesozoic. This study also has new insights into the genesis of Cu mineralization in the QHMB and further provides implications for future exploration.