dc.contributor.author
Asimi, Vahid
dc.contributor.author
Kumar, Abhishek Sampath
dc.contributor.author
Niskanen, Henri
dc.contributor.author
Riemenschneider, Christina
dc.contributor.author
Hetzel, Sara
dc.contributor.author
Naderi, Julian
dc.contributor.author
Fasching, Nina
dc.contributor.author
Popitsch, Niko
dc.contributor.author
Du, Manyu
dc.contributor.author
Kretzmer, Helene
dc.date.accessioned
2022-09-05T09:11:41Z
dc.date.available
2022-09-05T09:11:41Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/36161
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-35877
dc.description.abstract
Most endogenous retroviruses (ERVs) in mammals are incapable of retrotransposition; therefore, why ERV derepression is associated with lethality during early development has been a mystery. Here, we report that rapid and selective degradation of the heterochromatin adapter protein TRIM28 triggers dissociation of transcriptional condensates from loci encoding super-enhancer (SE)-driven pluripotency genes and their association with transcribed ERV loci in murine embryonic stem cells. Knockdown of ERV RNAs or forced expression of SE-enriched transcription factors rescued condensate localization at SEs in TRIM28-degraded cells. In a biochemical reconstitution system, ERV RNA facilitated partitioning of RNA polymerase II and the Mediator coactivator into phase-separated droplets. In TRIM28 knockout mouse embryos, single-cell RNA-seq analysis revealed specific depletion of pluripotent lineages. We propose that coding and noncoding nascent RNAs, including those produced by retrotransposons, may facilitate ‘hijacking’ of transcriptional condensates in various developmental and disease contexts.
en
dc.format.extent
39 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Gene regulation
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Hijacking of transcriptional condensates by endogenous retroviruses
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1038/s41588-022-01132-w
dcterms.bibliographicCitation.journaltitle
Nature Genetics
dcterms.bibliographicCitation.number
8
dcterms.bibliographicCitation.pagestart
1238
dcterms.bibliographicCitation.pageend
1247
dcterms.bibliographicCitation.volume
54
dcterms.bibliographicCitation.url
https://doi.org/10.1038/s41588-022-01132-w
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1546-1718
refubium.resourceType.provider
WoS-Alert