dc.contributor.author
Bartenschlager, Florian
dc.contributor.author
Klymiuk, Nikolai
dc.contributor.author
Weise, Christoph
dc.contributor.author
Kuropka, Benno
dc.contributor.author
Gruber, Achim D.
dc.contributor.author
Mundhenk, Lars
dc.date.accessioned
2022-06-20T12:37:21Z
dc.date.available
2022-06-20T12:37:21Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/35343
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-35059
dc.description.abstract
Species-specific diversities are particular features of mammalian chloride channel regulator, calcium activated (CLCA) genes. In contrast to four complex gene clusters in mammals, only two CLCA genes appear to exist in chickens. CLCA2 is conserved in both, while only the galline CLCA1 (gCLCA1) displays close genetic distance to mammalian clusters 1, 3 and 4. In this study, sequence analyses and biochemical characterizations revealed that gCLCA1 as a putative avian prototype shares common protein domains and processing features with all mammalian CLCA homologues. It has a transmembrane (TM) domain in the carboxy terminal region and its mRNA and protein were detected in the alimentary canal, where the protein was localized in the apical membrane of enterocytes, similar to CLCA4. Both mammals and birds seem to have at least one TM domain containing CLCA protein with complex glycosylation in the apical membrane of enterocytes. However, some characteristic features of mammalian CLCA1 and 3 including entire protein secretion and expression in cell types other than enterocytes seem to be dispensable for chicken. Phylogenetic analyses including twelve bird species revealed that avian CLCA1 and mammalian CLCA3 form clades separate from a major branch containing mammalian CLCA1 and 4. Overall, our data suggest that gCLCA1 and mammalian CLCA clusters 1, 3 and 4 stem from a common ancestor which underwent complex gene diversification in mammals but not in birds.
en
dc.format.extent
25 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Protein domains
en
dc.subject
Transfection
en
dc.subject
Membrane proteins
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Evolutionarily conserved properties of CLCA proteins 1, 3 and 4, as revealed by phylogenetic and biochemical studies in avian homologues
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e0266937
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0266937
dcterms.bibliographicCitation.journaltitle
PLoS ONE
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.volume
17
dcterms.bibliographicCitation.url
https://doi.org/10.1371/journal.pone.0266937
refubium.affiliation
Veterinärmedizin
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Tierpathologie

refubium.affiliation.other
Institut für Chemie und Biochemie

refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin finanziert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1932-6203
refubium.resourceType.provider
WoS-Alert