dc.contributor.author
Eichstädt, Bernadette
dc.contributor.author
Lederer, Sarah
dc.contributor.author
Trempel, Fabian
dc.contributor.author
Jiang, Xiyuan
dc.contributor.author
Guerra, Tiziana
dc.contributor.author
Waadt, Rainer
dc.contributor.author
Lee, Justin
dc.contributor.author
Liese, Anja
dc.contributor.author
Romeis, Tina
dc.date.accessioned
2022-02-07T10:48:24Z
dc.date.available
2022-02-07T10:48:24Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/33913
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-33632
dc.description.abstract
Upon pathogen recognition, a transient rise in cytoplasmic calcium levels is one of the earliest events in plants and a prerequisite for defense initiation and signal propagation from a local site to systemic plant tissues. However, it is unclear if calcium signaling differs in the context of priming: Do plants exposed to a first pathogen stimulus and have consequently established systemic acquired resistance (SAR) display altered calcium responses to a second pathogen stimulus? Several calcium indicator systems including aequorin, YC3.6 or R-GECO1 have been used to document local calcium responses to the bacterial flg22 peptide but systemic calcium imaging within a single plant remains a technical challenge. Here, we report on an experimental approach to monitor flg22-induced calcium responses in systemic leaves of primed plants. The calcium-dependent protein kinase CPK5 is a key calcium sensor and regulator of the NADPH oxidase RBOHD and plays a role in the systemic calcium-ROS signal propagation. We therefore compared flg22-induced cytoplasmic calcium changes in Arabidopsis wild-type, cpk5 mutant and CPK5-overexpressing plants (exhibiting constitutive priming) by introgressing the calcium indicator R-GECO1-mTurquoise that allows internal normalization through mTurquoise fluorescence. Aequorin-based analyses were included for comparison. Based on the R-GECO1-mTurquoise data, CPK5-OE appears to reinforce an “oscillatory-like” Ca2+ signature in flg22-treated local tissues. However, no change was observed in the flg22-induced calcium response in the systemic tissues of plants that had been pre-challenged by a priming stimulus – neither in wild-type nor in cpk5 or CPK5-OE-lines. These data indicate that the mechanistic manifestation of a plant immune memory in distal plant parts required for enhanced pathogen resistance does not include changes in rapid calcium signaling upstream of CPK5 but rather relies on downstream defense responses.
en
dc.format.extent
14 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
calcium signature
en
dc.subject
systemic signaling
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::580 Pflanzen (Botanik)::580 Pflanzen (Botanik)
dc.title
Plant Immune Memory in Systemic Tissue Does Not Involve Changes in Rapid Calcium Signaling
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
798230
dcterms.bibliographicCitation.doi
10.3389/fpls.2021.798230
dcterms.bibliographicCitation.journaltitle
Frontiers in Plant Science
dcterms.bibliographicCitation.volume
12
dcterms.bibliographicCitation.url
https://doi.org/10.3389/fpls.2021.798230
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Biologie / Dahlem Centre of Plant Sciences (DCPS)
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1664-462X
refubium.resourceType.provider
WoS-Alert