dc.contributor.author
Ayala-Torres, Carlos
dc.contributor.author
Krug, Susanne M.
dc.contributor.author
Rosenthal, Rita
dc.contributor.author
Fromm, Michael
dc.date.accessioned
2022-01-27T13:03:36Z
dc.date.available
2022-01-27T13:03:36Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/33738
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-33458
dc.description.abstract
Water transport in epithelia occurs transcellularly (aquaporins) and paracellularly (claudin-2, claudin-15). Recently, we showed that downregulated tricellulin, a protein of the tricellular tight junction (tTJ, the site where three epithelial cells meet), increased transepithelial water flux. We now check the hypothesis that another tTJ-associated protein, angulin-1 (alias lipolysis-stimulated lipoprotein receptor, LSR) is a direct negative actuator of tTJ water permeability depending on the tightness of the epithelium. For this, a tight and an intermediate-tight epithelial cell line, MDCK C7 and HT-29/B6, were stably transfected with CRISPR/Cas9 and single-guide RNA targeting angulin-1 and morphologically and functionally characterized. Water flux induced by an osmotic gradient using 4-kDa dextran caused water flux to increase in angulin-1 KO clones in MDCK C7 cells, but not in HT-29/B6 cells. In addition, we found that water permeability in HT-29/B6 cells was not modified after either angulin-1 knockout or tricellulin knockdown, which may be related to the presence of other pathways, which reduce the impact of the tTJ pathway. In conclusion, modulation of the tTJ by knockout or knockdown of tTJ proteins affects ion and macromolecule permeability in tight and intermediate-tight epithelial cell lines, while the transepithelial water permeability was affected only in tight cell lines.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
tricellular tight junction
en
dc.subject
paracellular water transport
en
dc.subject
tight epithelium
en
dc.subject
MDCK C7 cells
en
dc.subject
intermediate-tight epithelium
en
dc.subject
HT-29/B6 cells
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Angulin-1 (LSR) Affects Paracellular Water Transport, However Only in Tight Epithelial Cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
7827
dcterms.bibliographicCitation.doi
10.3390/ijms22157827
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
15
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
22
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
34360593
dcterms.isPartOf.eissn
1422-0067