dc.contributor.author
Klotz, Christian
dc.contributor.author
Radam, Elke
dc.contributor.author
Rausch, Sebastian
dc.contributor.author
Gosten-Heinrich, Petra
dc.contributor.author
Aebischer, Toni
dc.date.accessioned
2021-08-16T14:05:16Z
dc.date.available
2021-08-16T14:05:16Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/31642
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-31373
dc.description.abstract
Giardiasis in humans is a gastrointestinal disease transmitted by the potentially zoonotic Giardia duodenalis genotypes (assemblages) A and B. Small wild rodents such as mice and voles are discussed as potential reservoirs for G. duodenalis but are predominantly populated by the two rodent species Giardia microti and Giardia muris. Currently, the detection of zoonotic and non-zoonotic Giardia species and genotypes in these animals relies on cumbersome PCR and sequencing approaches of genetic marker genes. This hampers the risk assessment of potential zoonotic Giardia transmissions by these animals. Here, we provide a workflow based on newly developed real-time PCR schemes targeting the small ribosomal RNA multi-copy gene locus to distinguish G. muris, G. microti and G. duodenalis infections. For the identification of potentially zoonotic G. duodenalis assemblage types A and B, an established protocol targeting the single-copy gene 4E1-HP was used. The assays were specific for the distinct Giardia species or genotypes and revealed an analytical sensitivity of approximately one or below genome equivalent for the multi-copy gene and of about 10 genome equivalents for the single-copy gene. Retesting a biobank of small rodent samples confirmed the specificity. It further identified the underlying Giardia species in four out of 11 samples that could not be typed before by PCR and sequencing. The newly developed workflow has the potential to facilitate the detection of potentially zoonotic and non-zoonotic Giardia species in wild rodents.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Giardia spp.
en
dc.subject
wild rodents
en
dc.subject
molecular detection
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Real-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic Giardia spp. in Wild Rodents
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1610
dcterms.bibliographicCitation.doi
10.3390/microorganisms9081610
dcterms.bibliographicCitation.journaltitle
Microorganisms
dcterms.bibliographicCitation.number
8
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
9
dcterms.bibliographicCitation.url
https://doi.org/10.3390/microorganisms9081610
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Immunologie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2076-2607