dc.contributor.author
Frosini, Sian Marie
dc.contributor.author
Bond, Ross
dc.contributor.author
McCarthy, Alex J.
dc.contributor.author
Feudi, Claudia
dc.contributor.author
Schwarz, Stefan
dc.contributor.author
Lindsay, Jodi A.
dc.contributor.author
Loeffler, Anette
dc.date.accessioned
2021-02-08T16:23:49Z
dc.date.available
2021-02-08T16:23:49Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29553
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29297
dc.description.abstract
Transmission of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) between people and pets, and their co-carriage, are well-described. Potential exchange of antimicrobial resistance (AMR) genes amongst these staphylococci was investigated in vitro through endogenous bacteriophage-mediated transduction. Bacteriophages were UV-induced from seven donor isolates of canine (MRSP) and human (MRSA) origin, containing tet(M), tet(K), fusB or fusC, and lysates filtered. Twenty-seven tetracycline- and fusidic acid- (FA-) susceptible recipients were used in 122 donor-recipient combinations (22 tetracycline, 100 FA) across 415 assays (115 tetracycline, 300 FA). Bacteriophage lysates were incubated with recipients and presumed transductants quantified on antimicrobial-supplemented agar plates. Tetracycline resistance transduction from MRSP and MRSA to methicillin-susceptible S. pseudintermedius (MSSP) was confirmed by PCR in 15/115 assays. No FA-resistance transfer occurred, confirmed by negative fusB/fusC PCR, but colonies resulting from FA assays had high MICs (≥32 mg/L) and showed mutations in fusA, two at a novel position (F88L), nine at H457[Y/N/L]. Horizontal gene transfer of tetracycline-resistance confirms that resistance genes can be shared between coagulase-positive staphylococci from different hosts. Cross-species AMR transmission highlights the importance of good antimicrobial stewardship across humans and veterinary species to support One Health.
en
dc.format.extent
12 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
staphylococci
en
dc.subject
bacteriophage
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Genes on the Move: In Vitro Transduction of Antimicrobial Resistance Genes between Human and Canine Staphylococcal Pathogens
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2031
dcterms.bibliographicCitation.doi
10.3390/microorganisms8122031
dcterms.bibliographicCitation.journaltitle
Microorganisms
dcterms.bibliographicCitation.number
12
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
8
dcterms.bibliographicCitation.url
https://doi.org/10.3390/microorganisms8122031
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Mikrobiologie und Tierseuchen

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2076-2607