dc.contributor.author
Waldt, Natalie
dc.contributor.author
Seifert, Anke
dc.contributor.author
Demiray, Yunus Emre
dc.contributor.author
Devroe, Eric
dc.contributor.author
Turk, Benjamin E.
dc.contributor.author
Reichardt, Peter
dc.contributor.author
Mix, Charlie
dc.contributor.author
Reinhold, Annegret
dc.contributor.author
Freund, Christian
dc.contributor.author
Müller, Andreas J.
dc.contributor.author
Schraven, Burkhart
dc.contributor.author
Stork, Oliver
dc.contributor.author
Kliche, Stefanie
dc.date.accessioned
2019-05-03T10:20:55Z
dc.date.available
2019-05-03T10:20:55Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/24525
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-2289
dc.description.abstract
The integrin LFA-1 (CD11a/CD18) plays a critical role in the interaction of T cells with antigen presenting cells (APCs) to promote lymphocyte differentiation and proliferation. This integrin can be present either in a closed or in an open active conformation and its activation upon T-cell receptor (TCR) stimulation is a critical step to allow interaction with APCs. In this study we demonstrate that the serine/threonine kinase Ndr2 is critically involved in the initiation of TCR-mediated LFA-1 activation (open conformation) in T cells. Ndr2 itself becomes activated upon TCR stimulation and phosphorylates the intracellular integrin binding partner Filamin A (FLNa) at serine 2152. This phosphorylation promotes the dissociation of FLNa from LFA-1, allowing for a subsequent association of Talin and Kindlin-3 which both stabilize the open conformation of LFA-1. Our data suggest that Ndr2 activation is a crucial step to initiate TCR-mediated LFA-1 activation in T cells.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
inside-out signaling
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::572 Biochemie
dc.title
Filamin A Phosphorylation at Serine 2152 by the Serine/Threonine Kinase Ndr2 Controls TCR-Induced LFA-1 Activation in T Cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2852
dcterms.bibliographicCitation.doi
10.3389/fimmu.2018.02852
dcterms.bibliographicCitation.journaltitle
Frontiers in Immunology
dcterms.bibliographicCitation.volume
9
dcterms.bibliographicCitation.url
https://doi.org/10.3389/fimmu.2018.02852
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access