id,collection,dc.contributor.author,dc.date.accessioned,dc.date.available,dc.date.issued,dc.description.abstract[en],dc.format.extent,dc.identifier.uri,dc.language,dc.rights.uri,dc.subject.ddc,dc.subject[],dc.subject[en],dc.title,dc.type,dcterms.accessRights.openaire,dcterms.bibliographicCitation.articlenumber,dcterms.bibliographicCitation.doi,dcterms.bibliographicCitation.journaltitle,dcterms.bibliographicCitation.url,dcterms.bibliographicCitation.volume,refubium.affiliation,refubium.resourceType.isindependentpub "9fd117dd-1478-419e-b7e1-bc0b495dd3cf","fub188/16","Waldt, Natalie||Seifert, Anke||Demiray, Yunus Emre||Devroe, Eric||Turk, Benjamin E.||Reichardt, Peter||Mix, Charlie||Reinhold, Annegret||Freund, Christian||Müller, Andreas J.||Schraven, Burkhart||Stork, Oliver||Kliche, Stefanie","2019-05-03T10:20:55Z","2019-05-03T10:20:55Z","2018","The integrin LFA-1 (CD11a/CD18) plays a critical role in the interaction of T cells with antigen presenting cells (APCs) to promote lymphocyte differentiation and proliferation. This integrin can be present either in a closed or in an open active conformation and its activation upon T-cell receptor (TCR) stimulation is a critical step to allow interaction with APCs. In this study we demonstrate that the serine/threonine kinase Ndr2 is critically involved in the initiation of TCR-mediated LFA-1 activation (open conformation) in T cells. Ndr2 itself becomes activated upon TCR stimulation and phosphorylates the intracellular integrin binding partner Filamin A (FLNa) at serine 2152. This phosphorylation promotes the dissociation of FLNa from LFA-1, allowing for a subsequent association of Talin and Kindlin-3 which both stabilize the open conformation of LFA-1. Our data suggest that Ndr2 activation is a crucial step to initiate TCR-mediated LFA-1 activation in T cells.","16 S.","https://refubium.fu-berlin.de/handle/fub188/24525||http://dx.doi.org/10.17169/refubium-2289","eng","https://creativecommons.org/licenses/by/4.0/","500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::572 Biochemie","T cells","LFA-1||TCR||Ndr2||Filamin A||Talin||Kindlin-3||inside-out signaling","Filamin A Phosphorylation at Serine 2152 by the Serine/Threonine Kinase Ndr2 Controls TCR-Induced LFA-1 Activation in T Cells","Wissenschaftlicher Artikel","open access","2852","10.3389/fimmu.2018.02852","Frontiers in Immunology","https://doi.org/10.3389/fimmu.2018.02852","9","Biologie, Chemie, Pharmazie","no"