Radical Stability vs. Temporal Resolution of EPR-Spectroscopy on Biological Samples
Walker, Karolina A.; Unbehauen, Michael L.; Lohan, Silke B.; Saeidpour, Siavash; Meinke, Martina C.; Zimmer, Reinhold; Haag, Rainer
Datum der Freigabe:
Spin-labeling active compounds is a convenient way to prepare them for EPR spectroscopy with minimal alteration of the target molecule. In this study we present the labeling reaction of dexamethasone (Dx) with either TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy) or PCA (3-(carboxy)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy) with high yields. According to NMR data, both labels are attached at the primary hydroxy group of the steroid. In subsequent spin-stability measurements both compounds were applied onto HaCaT cells. When the signal of Dx-TEMPO decreased below the detection limit within 3 h, the signal of Dx-PCA remained stable for the same period of time.