The coherent superposition of states, in combination with the quantization of observables, represents one of the most fundamental features that mark the departure of quantum mechanics from the classical realm. Quantum coherence in many-body systems embodies the essence of entanglement and is an essential ingredient for a plethora of physical phenomena in quantum optics, quantum information, solid state physics, and nanoscale thermodynamics. In recent years, research on the presence and functional role of quantum coherence in biological systems has also attracted considerable interest. Despite the fundamental importance of quantum coherence, the development of a rigorous theory of quantum coherence as a physical resource has been initiated only recently. This Colloquium discusses and reviews the development of this rapidly growing research field that encompasses the characterization, quantification, manipulation, dynamical evolution, and operational application of quantum coherence.