dc.contributor.author
Nowak, Kathrin
dc.contributor.author
Fahr, Jakob
dc.contributor.author
Weber, Natalie
dc.contributor.author
Luebke-Becker, Antina
dc.contributor.author
Semmler, Torsten
dc.contributor.author
Weiss, Sabrina
dc.contributor.author
Mombouli, Jean-Vivien
dc.contributor.author
Wieler, Lothar H.
dc.contributor.author
Guenther, Sebastian
dc.contributor.author
Leendertz, Fabian H.
dc.contributor.author
Ewers, Christa
dc.date.accessioned
2018-06-08T10:52:11Z
dc.date.available
2017-09-15T08:44:31.705Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/21249
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24544
dc.description.abstract
Bats are suspected to be a reservoir of several bacterial and viral pathogens
relevant to animal and human health, but studies on Escherichia coli in these
animals are sparse. We investigated the presence of E. coli in tissue samples
(liver, lung and intestines) collected from 50 fruit bats of five different
species (Eidolon helvum, Epomops franqueti, Hypsignathus monstrosus,
Myonycteris torquata, Rousettus aegyptiacus) of two different areas in the
Republic of Congo between 2009 and 2010. To assess E. coli pathotypes and
phylogenetic relationships, we determined the presence of 59 virulence
associated genes and multilocus sequence types (STs). Isolates were further
tested for their susceptibility to several antimicrobial substances by agar
disk diffusion test and for the presence of an Extended-Spectrum Beta-
Lactamase phenotype. E. coli was detected in 60% of the bats analysed. The
diversity of E. coli strains was very high, with 37 different STs within 40
isolates. Occasionally, we detected sequence types (e.g. ST69, ST127, and
ST131) and pathotypes (e.g. ExPEC, EPEC and atypical EPEC), which are known
pathogens in human and/or animal infections. Although the majority of strains
were assigned to phylogenetic group B2 (46.2%), which is linked with the ExPEC
pathovar, occurrence of virulence-associated genes in these strains were
unexpectedly low. Due to this, and as only few of the E. coli isolates showed
intermediate resistance to certain antimicrobial substances, we assume a
rather naïve E. coli population, lacking contact to humans or domestic
animals. Future studies featuring in depth comparative whole genome sequence
analyses will provide insights into the microevolution of this interesting
strain collection.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Highly diverse and antimicrobial susceptible Escherichia coli display a naïve
bacterial population in fruit bats from the Republic of Congo
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 12 (2017), 7, Artikel Nr. e0178146
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0178146
dcterms.bibliographicCitation.url
http://doi.org/10.1371/journal.pone.0178146
refubium.affiliation
Biologie, Chemie, Pharmazie
de
refubium.mycore.fudocsId
FUDOCS_document_000000027972
refubium.note.author
Der Artikel wurde in einer reinen Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000008746
dcterms.accessRights.openaire
open access