dc.contributor.author
Ravn, Christen
dc.contributor.author
Tafin, Ulrika Furustrand
dc.contributor.author
Betrisey, Bertrand
dc.contributor.author
Overgaard, Soren
dc.contributor.author
Trampuz, Andrej
dc.date.accessioned
2018-06-08T10:40:50Z
dc.date.available
2017-01-20T13:22:24.226Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/20876
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-24175
dc.description.abstract
Background and purpose — Antibiotic treatment of patients before specimen
collection reduces the ability to detect organisms by culture. We investigated
the suppressive effect of antibiotics on the growth of non-adherent,
planktonic, and surface-related biofilm bacteria in vitro by using sonication
and microcalorimetry methods. Patients and methods — Biofilms of
Staphylococcus aureus, S. epidermidis, Escherichia coli, and Propionibacterium
acnes were formed on porous glass beads and exposed for 24 h to antibiotic
concentrations from 1 to 1,024 times the minimal inhibitory concentration
(MIC) of vancomycin, daptomycin, rifampin, flucloxacillin, or ciprofloxacin.
The beads were then sonicated to dislodge biofilm, followed by culture and
measurement of growth-related heat flow by microcalorimetry of the resulting
sonication fluid. Results — Vancomycin did not inhibit the heat flow of
staphylococci and P. acnes at concentrations ≤1,024 μg/mL, whereas
flucloxacillin at >128 μg/mL inhibited S. aureus. Daptomycin inhibited heat
flow of S. aureus, S. epidermidis, and P. acnes at lower concentrations
(32–128 times MIC, p < 0.001). Rifampin showed inconsistent results in
staphylococci due to random emergence of resistance, which was observed at
concentrations ≤1,024 times MIC (i.e. 8 μg/mL). Ciprofloxacin inhibited heat
flow of E. coli at ≥4 times MIC (i.e. ≥ 0.06 μg/mL). Interpretation — Whereas
time-dependent antibiotics (i.e. vancomycin and flucloxacillin) showed only
weak growth suppression, concentration-dependent drugs (i.e. daptomycin and
ciprofloxacin) had a strong suppressive effect on bacterial growth and reduced
the ability to detect planktonic and biofilm bacteria. Exposure to rifampin
rapidly caused emergence of resistance. Our findings indicate that
preoperative administration of antibiotics may have heterogeneous effects on
the ability to detect biofilm bacteria.
en
dc.rights.uri
http://www.actaorthop.org/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit
dc.title
Reduced ability to detect surface-related biofilm bacteria after antibiotic
exposure under in vitro conditions
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
Acta Orthopaedica. - 87 (2016), 6, S. 644-650
dcterms.bibliographicCitation.doi
10.1080/17453674.2016.1246795
dcterms.bibliographicCitation.url
http://dx.doi.org/10.1080/17453674.2016.1246795
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000026183
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000007563
dcterms.accessRights.openaire
open access