Title:
[NiFe]-hydrogenase maturation
Subtitle:
Isolation of a HypC–HypD complex carrying diatomic CO and CN− ligands
Author(s):
Soboh, Basem; Stripp, Sven T.; Muhr, Enrico; Granich, Claudia; Braussemann, Mario; Herzberg, Martin; Heberle, Joachim; Sawers, Gary R.
Year of publication:
2012
Available Date:
2014-09-03T11:15:58.463Z
Abstract:
The HypC and HypD maturases are required for the biosynthesis of the Fe(CN)2CO
cofactor in the large subunit of [NiFe]-hydrogenases. Using infrared
spectroscopy we demonstrate that an anaerobically purified, Strep-tagged HypCD
complex from Escherichia coli exhibits absorption bands characteristic of
diatomic CO and CN− ligands as well as CO2. Metal and sulphide analyses
revealed that along with the [4Fe–4S]2+ cluster in HypD, the complex has two
additional oxygen-labile Fe ions. We prove that HypD cysteine 41 is required
for the coordination of all three ligands. These findings suggest that the
HypCD complex carries minimally the Fe(CN)2CO cofactor.
Part of Identifier:
ISSN (print): 00145793
Keywords:
Hydrogenase
Iron
Diatomic ligand
Cyanide
Carbon monoxide
Infrared spectroscopy
DDC-Classification:
530 Physik
Publication Type:
Wissenschaftlicher Artikel
Also published in:
FEBS Letters. - 586 (2012), 21, S.3882-3887
URL of the Original Publication:
DOI of the Original Publication:
Department/institution:
Physik
Institut für Experimentalphysik
