dc.contributor.author
Abass, Elfadil
dc.contributor.author
Kang, Cholho
dc.contributor.author
Martinkovic, Franjo
dc.contributor.author
Semião-Santos, Saul J.
dc.contributor.author
Sundar, Shyam
dc.contributor.author
Walden, Peter
dc.contributor.author
Piarroux, Renaud
dc.contributor.author
Harith, Abdallah el
dc.contributor.author
Lohoff, Michael
dc.contributor.author
Steinhoff, Ulrich
dc.date.accessioned
2018-06-08T03:43:48Z
dc.date.available
2015-04-17T07:26:47.348Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/15803
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-19990
dc.description.abstract
Diagnostic tests for visceral leishmaniasis that are based on antigens of a
single Leishmania strain can have low diagnostic performance in regions where
heterologous parasites predominate. The aim of this study was to investigate
and compare the performance of five serological tests, based on different
Leishmania antigens, in three endemic countries for visceral leishmaniasis. A
total number of 231 sera of symptomatic and asymptomatic cases and controls
from three endemic regions of visceral leishmaniasis in East Sudan, North
India and South France were evaluated by following serological tests: rKLO8-
and rK39 ELISA, DAT (ITMA-DAT) and two rapid tests of rK39 (IT LEISH) and
rKE16 (Signal-KA). Overall, rKLO8- and rK39 ELISA were most sensitive in
immunocompetent patients from all endemic regions (96–100%) and the
sensitivity was reduced to 81.8% in HIV co-infected patients from France. Sera
of patients from India demonstrated significantly higher antibody responses to
rKLO8 and rK39 compared with sera from Sudan (p<0.0001) and France (p<0.0037).
Further, some Indian and Sudanese patients reacted better with rKLO8 than
rK39. Sensitivity of DAT (ITMA-DAT) was high in Sudan (94%) and India (92.3%)
but low in France being 88.5% and 54.5% for VL and VL/HIV patients,
respectively. In contrast, rapid tests displayed high sensitivity only in
patients from India (96.2%) but not Sudan (64–88%) and France (73.1–88.5% and
63.6–81.8% in VL and VL/HIV patients, respectively). While the sensitivity
varied, all tests showed high specificity in Sudan (96.7–100%) and India
(96.6%).Heterogeneity of Leishmania parasites which is common in many endemic
regions complicates the diagnosis of visceral leishmaniasis. Therefore, tests
based on homologous Leishmania antigens are required for particular endemic
regions to detect cases which are difficult to be diagnosed with currently
available tests.
en
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften
dc.title
Heterogeneity of Leishmania donovani Parasites Complicates Diagnosis of
Visceral Leishmaniasis
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation
PLoS ONE. - 10 (2015), 3, Artikel Nr. e0116408
dc.title.subtitle
Comparison of Different Serological Tests in Three Endemic Regions
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0116408
dcterms.bibliographicCitation.url
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116408
refubium.affiliation
Charité - Universitätsmedizin Berlin
de
refubium.mycore.fudocsId
FUDOCS_document_000000022210
refubium.note.author
Der Artikel wurde in einer Open-Access-Zeitschrift publiziert.
refubium.resourceType.isindependentpub
no
refubium.mycore.derivateId
FUDOCS_derivate_000000004774
dcterms.accessRights.openaire
open access