Brosi, Richard; Bittl, Robert; Engelhard, Christopher
Datum der Freigabe:
Flavoproteins often employ radical mechanisms in their enzymatic reactions.
This involves paramagnetic species, which can ideally be investigated with
electron paramagnetic resonance (EPR) spectroscopy. In this chapter we focus
on the example of flavin-based photoreceptors and discuss, how different EPR
methods have been used to extract information about the flavin radical’s
electronic state, its binding pocket, electron-transfer pathways, and about
the protein’s tertiary and quaternary structure.