dc.contributor.author
Helmes, Sabine
dc.date.accessioned
2018-06-07T19:18:34Z
dc.date.available
2000-12-14T00:00:00.649Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/5958
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-10157
dc.description
Die komplette Dissertation im pdf-Format (372.969 Bytes):
helme.pdf
dc.description.abstract
The occurence of HRS cells in cases of human CLL is a rare phenomenon. Until
recently, investigations on the origin of these HRS cells were restricted on
morphological description and immunohistochemical analysis. In this thesis,
the first molecular analysia of Ig gene rearrangements of a case of B-CLL with
HRS cells on the level of individual cells by single cell PCR is presented.
The analysis aimed at a more detailed understanding of the possible
relationship between Hodgkin´s disease and NHL.
The sequences of the Ig gene rearrangements of the NHL B cells were identical,
indicating clonality of these cells. An identical pattern was obtained for the
HRS cells. Comparing the sequences from the HRS cells and the B cells of the
NHL, a difference of on base was observed. Thus, the assumption that the two
cell populations are closely related is valid.
Due to the spareseness of important cases as studied in this thesis as a fresh
tissue biopsy, such single cell investigations are limited. A higher number of
comparable cases are conserved as formalin-fixed and paraffin-embedded samples
in different institutes interested in hematology. For that reason, we wanted
to transfer the single cell PCR technique established on fresh tissue to the
analysis of formalin-fixed and paraffin-embedded cells. Since DNA is degraded
by formalin, primers were established that bind to sequences in the FR2 and
FR3 of the V genes in order to generate PCR products shorter than those
obtained with primers binding to sequences in the FR1 of the genes in the Ig
heavy chain locus.
We could show that generally the detection of gene rearrangements in single
cells isolated from paraffin tissue is possible. However, compared to the
efficiency obtained using fresh tissue, the observed efficiency of 10% was
low.
en
dc.rights.uri
http://www.fu-berlin.de/sites/refubium/rechtliches/Nutzungsbedingungen
dc.subject
Hodgkin´s-disease
dc.subject
polymerase-chain-reaction
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Analyse von Hodgkin-Zellen in B-Zell chronischer lymphatischer Leukämie
mittels Einzelzell-PCR und Untersuchungen zur Etablierung der Einzelzell-PCR
von Zellen aus Formalin-fixierten, in Paraffin eingebetteten Biopsaten
dc.contributor.firstReferee
Univ.-Prof. Dr. G. Monreal
dc.contributor.furtherReferee
Univ.-Prof. Dr. M.-L. Hansmann / Köln
dc.date.accepted
1997-06-20
dc.date.embargoEnd
2001-02-07
dc.identifier.urn
urn:nbn:de:kobv:188-1998000521
dc.title.translated
Analysis of Hodgkin cells in B cell chronic lymphatic leukemia by single cell
PCR and experiments on the establishment of the single cell PCR on cells
isolated from formalin-fixed paraffin-embedded biopsies
en
refubium.affiliation
Veterinärmedizin
de
refubium.mycore.fudocsId
FUDISS_thesis_000000000057
refubium.mycore.transfer
http://www.diss.fu-berlin.de/1998/52/
refubium.mycore.derivateId
FUDISS_derivate_000000000057
dcterms.accessRights.dnb
free
dcterms.accessRights.openaire
open access