dc.contributor.author
Rincon-Arevalo, Hector
dc.contributor.author
Stefanski, Ana-Luisa
dc.contributor.author
Le, Tuan Anh
dc.contributor.author
Cases, Marcos
dc.contributor.author
Wiedemann, Annika
dc.contributor.author
Szelinski, Franziska
dc.contributor.author
Ritter, Jacob
dc.contributor.author
Dang, Van Duc
dc.contributor.author
Lino, Andreia C.
dc.contributor.author
Dörner, Thomas
dc.contributor.author
Schrezenmeier, Eva
dc.date.accessioned
2025-07-09T16:09:48Z
dc.date.available
2025-07-09T16:09:48Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/48192
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-47914
dc.description.abstract
Memory B cells (mBCs) are characterized by their long-term stability, fast reactivation, and capability to rapidly differentiate into antibody-secreting cells (ASCs). However, the role of T cells in the differentiation of mBCs, in contrast to naive B cells, remains to be delineated. We study the role of T cells in mBC responses, using CD40L stimulation and autologous T-B co-cultures. Our results showed that increased CD40L levels led to a selective increased proliferation of IgM+ mBC, which did not class-switched, resulting in higher frequencies of IgM+ ASCs and a lower frequency of IgG+ ASCs. The IgG+/IgA+ mBCs were unaffected. We further compared the transcription of immune-related genes in IgM+ and IgG+ pre-plasmablasts cultured at high (500 ng/mL) and low (50 ng/mL) CD40L levels. In response to increased CD40L levels, both populations exhibited a core response to genes related to activation (TRAF1, AKT3, CD69, and CD80). However, they differed in genes related to cytokine/chemokine/homing interactions (CCL3/4/17, LTA, NKX2-3, BCL2 and IL21R) and cell-cell interactions (HLADR, CD40, and ICOSL), which were largely confined to IgG+ cells. Our findings revealed that in co-cultures with a high T-ratio, the response was similar to that found in cultures with high CD40L levels. These results suggest that IgG+ mBCs have a greater capacity for proliferation and T cell interaction, and weaker migration capabilities, leading to a preference for an IgG response over IgM in the short term. This adaptable response could fine-tune the memory repertoire with different functions of IgG versus IgM mBCs.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
memory B cell
en
dc.subject
T:B co-stimulation
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Differential response of IgM and IgG memory B cell populations to CD40L: insights of T cell – memory B cell interactions
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1432045
dcterms.bibliographicCitation.doi
10.3389/fimmu.2024.1432045
dcterms.bibliographicCitation.journaltitle
Frontiers in Immunology
dcterms.bibliographicCitation.originalpublishername
Frontiers Media SA
dcterms.bibliographicCitation.volume
15
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
39050849
dcterms.isPartOf.eissn
1664-3224