dc.contributor.author
Jahnen, Johanna
dc.contributor.author
Hanke, Dennis
dc.contributor.author
Kadlec, Kristina
dc.contributor.author
Schwarz, Stefan
dc.contributor.author
Krüger-Haker, Henrike
dc.date.accessioned
2025-02-27T13:13:28Z
dc.date.available
2025-02-27T13:13:28Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/46694
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-46408
dc.description.abstract
Background/Objectives: Pasteurella multocida commonly colonizes the bovine respiratory tract and can occasionally cause intramammary infections. Here, eight P. multocida isolates from clinical cases of bovine mastitis were investigated for their molecular characteristics as well as phenotypic and genotypic antimicrobial resistance (AMR) properties. Methods: The isolates originated from quarter milk samples obtained in Germany for diagnostic purposes. Antimicrobial susceptibility testing (AST) by broth microdilution was performed according to the Clinical and Laboratory Standards Institute. Closed whole-genome sequences were generated by hybrid assembly of Illumina MiSeq short-reads and Oxford Nanopore MinION long-reads, followed by consecutive sequence analysis. Results: The P. multocida isolates belonged either to capsular:lipopolysaccharide type A:3 (n = 7) or A:6 (n = 1), and multi-locus sequence types 1 (n = 7) or 7 (n = 1). Seven isolates carried AMR genes, such as mef(C), mph(G), strA, strB, aphA1, aadA31, tet(H), tet(Y), floR, catA3, and sul2, as part of an integrative and conjugative element (ICE). These mobile genetic elements, 58,382–78,401 bp in size, were highly similar to the ICEs Tn7406 or Tn7407 that have been previously described in bovine Mannheimia haemolytica and P. multocida, respectively. Moreover, the isolates showed elevated minimal inhibitory concentrations corresponding to the identified AMR determinants. Conclusions: Molecular typing and ICE organization suggest the bovine respiratory tract as reservoir of the investigated mastitis-associated P. multocida. Horizontal cross-genus transfer of multidrug-resistance-mediating ICEs seems to occur under in vivo conditions among different pathogens from cattle in Germany, which underlines the importance of pathogen identification followed by AST for successful bovine mastitis therapy.
en
dc.format.extent
19 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
diagnostic sample
en
dc.subject
Pasteurellaceae
en
dc.subject
antimicrobial resistance surveillance
en
dc.subject
broth microdilution
en
dc.subject
whole-genome sequencing (WGS)
en
dc.subject
mobile genetic elements
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Antimicrobial Resistance in Pasteurella multocida Isolates from Bovine Mastitis Can Be Associated with Multidrug-Resistance-Mediating Integrative and Conjugative Elements (ICEs)
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
153
dcterms.bibliographicCitation.doi
10.3390/antibiotics14020153
dcterms.bibliographicCitation.journaltitle
Antibiotics
dcterms.bibliographicCitation.number
2
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
14
dcterms.bibliographicCitation.url
https://doi.org/10.3390/antibiotics14020153
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Mikrobiologie und Tierseuchen

refubium.affiliation.other
Tiermedizinisches Zentrum für Resistenzforschung (TZR)
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
de
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2079-6382