dc.contributor.author
Awad, Karem
dc.contributor.author
Barmeyer, Christian
dc.contributor.author
Bojarski, Christian
dc.contributor.author
Nagel, Oliver
dc.contributor.author
Lee, In-Fah M.
dc.contributor.author
Schweiger, Michal R.
dc.contributor.author
Schulzke, Jörg-Dieter
dc.contributor.author
Bücker, Roland
dc.date.accessioned
2024-07-03T08:40:53Z
dc.date.available
2024-07-03T08:40:53Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/44077
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-43787
dc.description.abstract
Background: In patients with diarrhea-predominant irritable bowel syndrome (IBS-D), the diarrheal mechanisms are largely unknown, and they were examined in this study on colon biopsies. Methods: Electrophysiological measurements were used for monitoring functional changes in the diarrheic colon specimens. In parallel, tight junction protein expression was analyzed by Western blot and confocal laser-scanning microscopy, and signaling pathway analysis was performed using RNA sequencing and bioinformatics. Results: Epithelial resistance was decreased, indicating an epithelial leak flux diarrheal mechanism with a molecular correlate of decreased claudin-1 expression, while induction of active anion secretion and impairment of active sodium absorption via the epithelial sodium channel, ENaC, were not detected. The pathway analysis revealed activation of barrier-affecting cytokines TNF-alpha, IFN-gamma, IL-1 beta and IL-4. Conclusions: Barrier dysfunction as a result of epithelial tight junction changes plays a role in IBS-D as a pathomechanism inducing a leak flux type of diarrhea.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
irritable bowel syndrome
en
dc.subject
intestinal barrier function
en
dc.subject
tight junctions
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Epithelial Barrier Dysfunction in Diarrhea-Predominant Irritable Bowel Syndrome (IBS-D) via Downregulation of Claudin-1
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2846
dcterms.bibliographicCitation.doi
10.3390/cells12242846
dcterms.bibliographicCitation.journaltitle
Cells
dcterms.bibliographicCitation.number
24
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
12
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
38132165
dcterms.isPartOf.eissn
2073-4409