dc.contributor.author
Tam, Nicky W.
dc.contributor.author
Schullian, Otto
dc.contributor.author
Cipitria, Amaia
dc.contributor.author
Dimova, Rumiana
dc.date.accessioned
2024-06-20T10:54:10Z
dc.date.available
2024-06-20T10:54:10Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/43904
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-43614
dc.description.abstract
The diffusion of extracellular vesicles and liposomes in vivo is affected by different tissue environmental conditions and is of great interest in the development of liposome-based therapeutics and drug-delivery systems. Here, we use a bottom-up biomimetic approach to better isolate and study steric and electrostatic interactions and their influence on the diffusivity of synthetic large unilamellar vesicles in hydrogel environments. Single-particle tracking of these extracellular vesicle-like particles in agarose hydrogels as an extracellular matrix model shows that membrane deformability and surface charge affect the hydrogel pore spaces that vesicles have access to, which determines overall diffusivity. Moreover, we show that passivation of vesicles with PEGylated lipids, as often used in drug-delivery systems, enhances diffusivity, but that this effect cannot be fully explained with electrostatic interactions alone. Finally, we compare our experimental findings with existing computational and theoretical work in the field to help explain the nonspecific interactions between diffusing particles and gel matrix environments.
en
dc.format.extent
13 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
membrane-matrix interactions
en
dc.subject
lipid vesicles
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
dc.title
Nonspecific membrane-matrix interactions influence diffusivity of lipid vesicles in hydrogels
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1016/j.bpj.2024.02.005
dcterms.bibliographicCitation.journaltitle
Biophysical Journal
dcterms.bibliographicCitation.number
5
dcterms.bibliographicCitation.pagestart
638
dcterms.bibliographicCitation.pageend
650
dcterms.bibliographicCitation.volume
123
dcterms.bibliographicCitation.url
https://doi.org/10.1016/j.bpj.2024.02.005
refubium.affiliation
Physik
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1542-0086
refubium.resourceType.provider
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