dc.contributor.author
Polewski, Lukasz
dc.contributor.author
Moon, Eunjin
dc.contributor.author
Zappe, Andreas
dc.contributor.author
Götze, Michael
dc.contributor.author
Szekeres, Gergő Péter
dc.contributor.author
Roth, Christian
dc.contributor.author
Pagel, Kevin
dc.date.accessioned
2024-06-26T09:47:02Z
dc.date.available
2024-06-26T09:47:02Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/43485
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-43202
dc.description.abstract
Glycosaminoglycans (GAGs) are linear and acidic polysaccharides. They are ubiquitous molecules, which are involved in a wide range of biological processes. Despite being structurally simple at first glance, with a repeating backbone of alternating hexuronic acid and hexosamine dimers, GAGs display a highly complex structure, which predominantly results from their heterogeneous sulfation patterns. The commonly applied method for compositional analysis of all GAGs is "disaccharide analysis." In this process, GAGs are enzymatically depolymerized into disaccharides, derivatized with a fluorescent label, and then analysed through liquid chromatography. The limiting factor in the high throughput analysis of GAG disaccharides is the time-consuming liquid chromatography. To address this limitation, we here utilized trapped ion mobility-mass spectrometry (TIM-MS) for the separation of isomeric GAG disaccharides, which reduces the measurement time from hours to a few minutes. A full set of disaccharides comprises twelve structures, with eight possessing isomers. Most disaccharides cannot be differentiated by TIM-MS in underivatized form. Therefore, we developed chemical modifications to reduce sample complexity and enhance differentiability. Quantification is performed using stable isotope labelled standards, which are easily available due to the nature of the performed modifications.
en
dc.format.extent
8 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc/4.0/
dc.subject
glycosaminoglycans
en
dc.subject
disaccharide analysis
en
dc.subject
trapped ion mobility spectrometry
en
dc.subject
quantification
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.title
Ion Mobility Mass Spectrometry-based Disaccharide Analysis of Glycosaminoglycans
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e202400783
dcterms.bibliographicCitation.doi
10.1002/chem.202400783
dcterms.bibliographicCitation.journaltitle
Chemistry – A European Journal
dcterms.bibliographicCitation.number
35
dcterms.bibliographicCitation.volume
30
dcterms.bibliographicCitation.url
https://doi.org/10.1002/chem.202400783
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie

refubium.funding
DEAL Wiley
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1521-3765