dc.contributor.author
Kunzler, Michael
dc.contributor.author
Schlechter, Rudolf O.
dc.contributor.author
Schreiber, Lukas
dc.contributor.author
Remus-Emsermann, Mitja N. P.
dc.date.accessioned
2024-05-07T10:29:43Z
dc.date.available
2024-05-07T10:29:43Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/43474
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-43191
dc.description.abstract
Here, we demonstrate the beneficial effect of surfactant-producing pseudomonads on Pantoea eucalypti 299R. We conducted a series of experiments in environments of increasing complexity. P. eucalypti 299R (Pe299R), and Pseudomonas sp. FF1 (Pff1) or Pe299R and surfactant-production deficient Pseudomonas sp. FF1::ΔviscB (Pff1ΔviscB) were co-inoculated in broth, on swarming agar plates, and on plants. In broth, there were no differences in the growth dynamics of Pe299R when growing in the presence of Pff1 or Pff1ΔviscB. By contrast, on swarming agar plates, Pe299R was able to co-swarm with Pff1 which led to a significant increase in Pe299R biomass compared to Pe299R growing with Pff1ΔviscB or in monoculture. Finally in planta, and using the single-cell bioreporter for reproductive success (CUSPER), we found a temporally distinct beneficial effect of Pff1 on co-inoculated Pe299R subpopulations that did not occur in the presence of Pff1ΔviscB. We tested three additional surfactant-producing pseudomonads and their respective surfactant knockout mutants on PE299R on swarming agar showing similar results. This led us to propose a model for the positive effect of surfactant production during leaf colonization. Our results indicate that co-motility might be common during leaf colonization and adds yet another facet to the already manyfold roles of surfactants.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Fluorescent proteins
en
dc.subject
Reproductive success
en
dc.subject
Bacteria-bacteria interactions
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Hitching a Ride in the Phyllosphere: Surfactant Production of Pseudomonas spp. Causes Co-swarming of Pantoea eucalypti 299R
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
62
dcterms.bibliographicCitation.doi
10.1007/s00248-024-02381-4
dcterms.bibliographicCitation.journaltitle
Microbial Ecology
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.volume
87
dcterms.bibliographicCitation.url
https://doi.org/10.1007/s00248-024-02381-4
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Biologie

refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1432-184X