dc.contributor.author
Said, Nelly
dc.contributor.author
Finazzo, Mark
dc.contributor.author
Hilal, Tarek
dc.contributor.author
Wang, Bing
dc.contributor.author
Selinger, Tim Luca
dc.contributor.author
Gjorgjevikj, Daniela
dc.contributor.author
Artsimovitch, Irina
dc.contributor.author
Wahl, Markus C.
dc.date.accessioned
2024-05-06T12:30:45Z
dc.date.available
2024-05-06T12:30:45Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/43455
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-43172
dc.description.abstract
Transcription termination factor ρ is a hexameric, RNA-dependent NTPase that can adopt active closed-ring and inactive open-ring conformations. The Sm-like protein Rof, a homolog of the RNA chaperone Hfq, inhibits ρ-dependent termination in vivo but recapitulation of this activity in vitro has proven difficult and the precise mode of Rof action is presently unknown. Here, our cryo-EM structures of ρ-Rof and ρ-RNA complexes show that Rof undergoes pronounced conformational changes to bind ρ at the protomer interfaces, undercutting ρ conformational dynamics associated with ring closure and occluding extended primary RNA-binding sites that are also part of interfaces between ρ and RNA polymerase. Consistently, Rof impedes ρ ring closure, ρ-RNA interactions and ρ association with transcription elongation complexes. Structure-guided mutagenesis coupled with functional assays confirms that the observed ρ-Rof interface is required for Rof-mediated inhibition of cell growth and ρ-termination in vitro. Bioinformatic analyses reveal that Rof is restricted to Pseudomonadota and that the ρ-Rof interface is conserved. Genomic contexts of rof differ between Enterobacteriaceae and Vibrionaceae, suggesting distinct modes of Rof regulation. We hypothesize that Rof and other cellular anti-terminators silence ρ under diverse, but yet to be identified, stress conditions when unrestrained transcription termination by ρ may be detrimental.
en
dc.format.extent
18 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Bacterial structural biology
en
dc.subject
Cryoelectron microscopy
en
dc.subject
Transcription
en
dc.subject
Transcription factors
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Sm-like protein Rof inhibits transcription termination factor ρ by binding site obstruction and conformational insulation
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
3186
dcterms.bibliographicCitation.journaltitle
Nature Communications
dcterms.bibliographicCitation.volume
15
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
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refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2041-1723