dc.contributor.author
Koçak, Alen
dc.contributor.author
Homer, Amal K.
dc.contributor.author
Feida, Antonia
dc.contributor.author
Telschow, Florian
dc.contributor.author
Gorenflos López, Jacob L.
dc.contributor.author
Baydaroğlu, Cihan
dc.contributor.author
Gradzielski, Michael
dc.contributor.author
Hackenberger, Christian P. R.
dc.contributor.author
Alexiev, Ulrike
dc.contributor.author
Seitz, Oliver
dc.date.accessioned
2024-05-06T11:12:53Z
dc.date.available
2024-05-06T11:12:53Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/43449
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-43166
dc.description.abstract
We show that covalent labelling of sialic acids on live cell surfaces or mucin increases the fluorescence of the fluorescence molecular rotors (FMRs) CCVJ, Cy3 and thioazole orange, enabling wash-free imaging of cell surfaces. Dual labelling with an FMR and an environmentally insensitive dye allows detection of changes that occur, for example, when cross-linking is altered.
en
dc.format.extent
4 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
covalent labelling
en
dc.subject
sialic acids
en
dc.subject
live cell surfaces
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.title
Fluorogenic cell surface glycan labelling with fluorescence molecular rotor dyes and nucleic acid stains
dc.type
Wissenschaftlicher Artikel
dc.date.updated
2024-05-01T02:58:57Z
dcterms.bibliographicCitation.doi
10.1039/d4cc00884g
dcterms.bibliographicCitation.journaltitle
Chemical Communications
dcterms.bibliographicCitation.number
36
dcterms.bibliographicCitation.originalpublishername
The Royal Society of Chemistry
dcterms.bibliographicCitation.pagestart
4785
dcterms.bibliographicCitation.pageend
4788
dcterms.bibliographicCitation.volume
60
dcterms.bibliographicCitation.url
https://doi.org/10.1039/D4CC00884G
refubium.affiliation
Physik
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1359-7345
dcterms.isPartOf.eissn
1364-548X
refubium.resourceType.provider
DeepGreen