dc.contributor.author
Elati, Khawla
dc.contributor.author
Tajeri, Shahin
dc.contributor.author
Mugo, Robert M.
dc.contributor.author
Obara, Isaiah
dc.contributor.author
Darghouth, Mohamed Aziz
dc.contributor.author
Zweygarth, Erich
dc.contributor.author
Menzo Nijhof, Ard
dc.date.accessioned
2024-03-01T08:29:33Z
dc.date.available
2024-03-01T08:29:33Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/42591
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-42315
dc.description.abstract
Theileria annulata is a protozoan parasite with a complex life cycle involving a bovine host and a tick vector. It is transmitted by Hyalomma ticks and is the causative agent of tropical theileriosis, a debilitating and often fatal disease in southern Europe, northern Africa and large parts of Asia. Understanding the biology of different life cycle stages is critical for the control of tropical theileriosis and requires the use of experimental animals which poses an ethical concern. We present for the first time the in vitro infection of red blood cells (RBCs) with T. annulata differentiated schizonts. The Ankara cell line of T. annulata was cultured at 41 °C for nine days to induce merogony and subsequently incubated with purified RBCs for one to three days. Percentage of parasitized erythrocyte (PPE) over the short culture period was estimated by Giemsa staining (0.007–0.01%), Flow cytometry activated sorting (FACS) (0.02–1.1%) and observation of FACS sorted cells by confocal microscopy (0.05–0.4%). There was a significant difference in the PPE between FACS and the two other techniques (one-way ANOVA followed by Tukey test, P = 0.004) but no significant difference was observed between the confocal imaging and Giemsa staining methods (ANOVA one-way followed by Tukey test, P = 0.06). Importantly, all three complementary methods confirmed the invasion of RBCs by T. annulata merozoites in vitro. Although the experimental conditions will require further optimization to increase the PPE, the in vitro infection of RBCs by T. annulata merozoites is pivotal in paving the way for the eventual completion of the T. annulata life cycle in vitro when combined with artificial tick feeding.
en
dc.format.extent
9 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Theileria annulata
en
dc.subject
In vitro infection
en
dc.subject
Bovine erythrocytes
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
In vitro infection of bovine erythrocytes with Theileria annulata merozoites as a key step in completing the T. annulata life cycle in vitro
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
3647
dcterms.bibliographicCitation.doi
10.1038/s41598-024-54327-y
dcterms.bibliographicCitation.journaltitle
Scientific Reports
dcterms.bibliographicCitation.volume
14
dcterms.bibliographicCitation.url
https://doi.org/10.1038/s41598-024-54327-y
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Parasitologie und Tropenveterinärmedizin
refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2045-2322