dc.contributor.author
Gavrilović, Ivana
dc.contributor.author
Wüst, Bernhard
dc.contributor.author
Danaceau, Jonathan
dc.contributor.author
Braidman, Emmanuelle
dc.contributor.author
de la Torre, Xavier
dc.contributor.author
Botrè, Francesco
dc.contributor.author
Parr, Maria Kristina
dc.contributor.author
Cowan, David
dc.date.accessioned
2024-07-29T11:28:36Z
dc.date.available
2024-07-29T11:28:36Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/42459
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-42184
dc.description.abstract
Supercritical fluid chromatography-mass spectrometry (SFC-MS) has proved to be a beneficial tool for sample analysis for a wide variety of compounds and, as such, has recently gained the attention of the anti-doping community. We have tested the applicability of SFC-MS for routine doping control analysing approximately 3 × 1000 identical anti-doping samples utilising SFC-MS instruments from three different vendors: Agilent Technologies, Waters Corporation and Shimadzu Corporation. A ‘dilute and inject’ approach either without or after hydrolysis of glucuronide metabolites was applied. Most of the compounds included in our study demonstrated excellent chromatography, whereas some showed co-elution with endogenous interferences requiring MS discrimination. Retention times typically were very stable within batches (%CV ≤ 0.5%), although this appeared to be analyte and column dependent. Chromatographic peak shape was good (symmetrical) and stable over the period of the testing without any change of column. Our results suggest that SFC-MS is a sensitive, reproducible and robust analytical tool ready to be used in anti-doping laboratories alongside the currently applied techniques such as gas and liquid chromatography coupled to mass spectrometry. Even if instruments are designed slightly differently, all three setups demonstrated their fitness for the purpose in anti-doping testing.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
doping control
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.title
Routine application of SFC-MS in doping control: Analysis of 3 × 1000 urine samples using three different SFC-MS instruments
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1002/dta.3652
dcterms.bibliographicCitation.journaltitle
Drug Testing and Analysis
dcterms.bibliographicCitation.number
7
dcterms.bibliographicCitation.pagestart
726
dcterms.bibliographicCitation.pageend
736
dcterms.bibliographicCitation.volume
16
dcterms.bibliographicCitation.url
https://doi.org/10.1002/dta.3652
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Pharmazie
refubium.funding
DEAL Wiley
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1942-7611