dc.contributor.author
Elati, Khawla
dc.contributor.author
Benyedem, Hayet
dc.contributor.author
Fukatsu, Kohsuke
dc.contributor.author
Hoffmann-Köhler, Peggy
dc.contributor.author
Mhadhbi, Moez
dc.contributor.author
Bakırcı, Serkan
dc.contributor.author
Bilgin Bilgiç, Hüseyin
dc.contributor.author
Karagenç, Tülin
dc.contributor.author
Aziz Darghouth, Mohamed
dc.contributor.author
Nijhof, Ard M.
dc.date.accessioned
2024-02-01T12:41:13Z
dc.date.available
2024-02-01T12:41:13Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/42250
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-41976
dc.description.abstract
Ticks are blood-sucking ectoparasites and can transmit various pathogens of medical and veterinary relevance. The life cycle of ticks can be completed under laboratory conditions on experimental animals, but the artificial feeding of ticks has attracted increased interest as an alternative method. This study represents the first report on the successful in vitro feeding of all life stages of two-host tick species, Hyalomma scupense and Hyalomma excavatum, and the three-host tick Hyalomma dromedarii. The attachment and engorgement rates of adults were 84% (21/25) and 76% (19/25) for H. scupense females. For adult H. excavatum and H. dromedarii, 70% (21/30) and 34.4% (11/32) of the females attached and all attached females successfully fed to repletion. The oviposition rates of the artificially fed females were 36.4%, 57.1% and 63.1% for H. dromedarii, H. excavatum and H. scupense, respectively, with a reproductive efficiency index varying between 44.3 and 60.7%. For the larvae, the attachment and engorgement rates were 44.2% (313/708) and 42.8% (303/708) for H. dromedarii, 70.5% (129/183) and 56.8% (104/183) for H. excavatum and 92.6% (113/122) and 55.7% (68/122) for H. scupense. The attachment and engorgement rates for the nymphs were 90.2% (129/143) and 47.6% (68/143) for H. dromedarii, 66.7% (34/51) and 41.2% (21/51) for H. excavatum, and 44.1% (30/68) and 36.8% (25/68) for H. scupense. Molting rates of the immature stages varied between 71.3% (216/303) and 100% (68/68) for the larvae and between 61.9% (13/21) and 96% (24/25) for the nymphs. The successful in vitro feeding of all stages of the three Hyalomma species makes this method a valuable tool for tick research, with potential applications in studies on the pathogens transmitted by these tick species such as Theileria annulata.
en
dc.format.extent
12 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Biological techniques
en
dc.subject
Biotechnology
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
In vitro feeding of all life stages of two-host Hyalomma excavatum and Hyalomma scupense and three-host Hyalomma dromedarii ticks
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
444
dcterms.bibliographicCitation.doi
10.1038/s41598-023-51052-w
dcterms.bibliographicCitation.journaltitle
Scientific Reports
dcterms.bibliographicCitation.volume
14
dcterms.bibliographicCitation.url
https://doi.org/10.1038/s41598-023-51052-w
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Parasitologie und Tropenveterinärmedizin
refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2045-2322