dc.contributor.author
Sander, Anna-Lena
dc.contributor.author
Moreira-Soto, Andres
dc.contributor.author
Yordanov, Stoian
dc.contributor.author
Toplak, Ivan
dc.contributor.author
Balboni, Andrea
dc.contributor.author
Ameneiros, Ramón Seage
dc.contributor.author
Corman, Victor
dc.contributor.author
Drosten, Christian
dc.contributor.author
Drexler, Jan Felix
dc.date.accessioned
2023-12-05T15:19:38Z
dc.date.available
2023-12-05T15:19:38Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/41754
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-41474
dc.description.abstract
The furin cleavage site (FCS) in SARS-CoV-2 is unique within the Severe acute respiratory syndrome-related coronavirus (SrC) species. We re-assessed diverse SrC from European horseshoe bats and analyzed the spike-encoding genomic region harboring the FCS in SARS-CoV-2. We reveal molecular features in SrC such as purine richness and RNA secondary structures that resemble those required for FCS acquisition in avian influenza viruses. We discuss the potential acquisition of FCS through molecular mechanisms such as nucleotide substitution, insertion, or recombination, and show that a single nucleotide exchange in two European bat-associated SrC may suffice to enable furin cleavage. Furthermore, we show that FCS occurrence is variable in bat- and rodent-borne counterparts of human coronaviruses. Our results suggest that furin cleavage sites can be acquired in SrC via conserved molecular mechanisms known in other reservoir-bound RNA viruses and thus support a natural origin of SARS-CoV-2.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Furin cleavage
en
dc.subject
Genomic determinants
en
dc.subject
European SARS-related bat coronaviruses
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Genomic determinants of Furin cleavage in diverse European SARS-related bat coronaviruses
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
491
dcterms.bibliographicCitation.doi
10.1038/s42003-022-03421-w
dcterms.bibliographicCitation.journaltitle
Communications Biology
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
Springer Nature
dcterms.bibliographicCitation.volume
5
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
Springer Nature DEAL
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
35637279
dcterms.isPartOf.eissn
2399-3642