dc.contributor.author
Jhelum, Hina
dc.contributor.author
Bender, Martin
dc.contributor.author
Reichart, Bruno
dc.contributor.author
Mokelke, Maren
dc.contributor.author
Radan, Julia
dc.contributor.author
Neumann, Elisabeth
dc.contributor.author
Krabben, Ludwig
dc.contributor.author
Abicht, Jan-Michael
dc.contributor.author
Kaufer, Benedikt
dc.contributor.author
Längin, Matthias
dc.contributor.author
Denner, Joachim
dc.date.accessioned
2023-08-25T12:45:48Z
dc.date.available
2023-08-25T12:45:48Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/40590
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-40311
dc.description.abstract
Xenotransplantation, like allotransplantation, is usually associated with microchimerism, i.e., the presence of cells from the donor in the recipient. Microchimerism was reported in first xenotransplantation trials in humans, as well as in most preclinical trials in nonhuman primates (for review, see Denner, Viruses 2023, 15, 190). When using pigs as xenotransplantation donors, their cells contain porcine endogenous retroviruses (PERVs) in their genome. This makes it difficult to discriminate between microchimerism and PERV infection of the recipient. Here, we demonstrate the appropriate virological methods to be used for the identification of microchimerism, first by screening for porcine cellular genes, and then how to detect infection of the host. Using porcine short interspersed nuclear sequences (SINEs), which have hundreds of thousands of copies in the pig genome, significantly increased the sensitivity of the screening for pig cells. Second, absence of PERV RNA demonstrated an absence of viral genomic RNA or expression as mRNA. Lastly, absence of antibodies against PERV proteins conclusively demonstrated an absence of a PERV infection. When applying these methods for analyzing baboons after pig heart transplantation, microchimerism could be demonstrated and infection excluded in all animals. These methods can be used in future clinical trials.
en
dc.format.extent
12 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
xenotransplantation
en
dc.subject
porcine endogenous retroviruses
en
dc.subject
microchimerism
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik
dc.title
Evidence for Microchimerism in Baboon Recipients of Pig Hearts
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1618
dcterms.bibliographicCitation.doi
10.3390/v15071618
dcterms.bibliographicCitation.journaltitle
Viruses
dcterms.bibliographicCitation.number
7
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
15
dcterms.bibliographicCitation.url
https://doi.org/10.3390/v15071618
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Virologie
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refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1999-4915