Diabetic pregnancy as a novel risk factor for cardiac dysfunction in the offspring—the heart as a target for fetal programming in rats

Abstract

Aims/hypothesis: The impact of diabetic pregnancy has been investigated extensively regarding offspring metabolism; however, little is known about the influence on the heart. We aimed to characterise the effects of a diabetic pregnancy on male adult offspring cardiac health after feeding a high-fat diet in an established transgenic rat model.

Methods: We applied our rat model for maternal type 2 diabetes characterised by maternal insulin resistance with hyperglycaemia and hyperinsulinaemia. Diabetes was induced preconceptionally via doxycycline-induced knock down of the insulin receptor in transgenic rats. Male wild-type offspring of diabetic and normoglycaemic pregnancies were raised by foster mothers, followed up into adulthood and subgroups were challenged by a high-fat diet. Cardiac phenotype was assessed by innovative speckle tracking echocardiography, circulating factors, immunohistochemistry and gene expression in the heart.

Results: When feeding normal chow, we did not observe differences in cardiac function, gene expression and plasma brain natriuretic peptide between adult diabetic or normoglycaemic offspring. Interestingly, when being fed a high-fat diet, adult offspring of diabetic pregnancy demonstrated decreased global longitudinal (-14.82 +/- 0.59 vs -16.60 +/- 0.48%) and circumferential strain (-23.40 +/- 0.57 vs -26.74 +/- 0.34%), increased relative wall thickness (0.53 +/- 0.06 vs 0.37 +/- 0.02), altered cardiac gene expression, enlarged cardiomyocytes (106.60 +/- 4.14 vs 87.94 +/- 1.67 gm), an accumulation of immune cells in the heart (10.27 +/- 0.30 vs 6.48 +/- 0.48 per fov) and higher plasma brain natriuretic peptide levels (0.50 +/- 0.12 vs 0.12 +/- 0.03 ng/ml) compared with normoglycaemic offspring on a high-fat diet. Blood pressure, urinary albumin, blood glucose and body weight were unaltered between groups on a high-fat diet.

Conclusions/interpretation: Diabetic pregnancy in rats induces cardiac dysfunction, left ventricular hypertrophy and altered pro inflammatory status in adult offspring only after a high-fat diet. A diabetic pregnancy itself was not sufficient to impair myocardial function and gene expression in male offspring later in life. This suggests that a postnatal high-fat diet is important for the development of cardiac dysfunction in rat offspring after diabetic pregnancy. Our data provide evidence that a diabetic pregnancy is a novel cardiac risk factor that becomes relevant when other challenges, such as a high-fat diet, are present.