dc.contributor.author
Griebel, Thomas
dc.contributor.author
Lapin, Dmitry
dc.contributor.author
Locci, Federica
dc.contributor.author
Kracher, Barbara
dc.contributor.author
Bautor, Jaqueline
dc.contributor.author
Concia, Lorenzo
dc.contributor.author
Benhamed, Moussa
dc.contributor.author
Parker, Jane E.
dc.date.accessioned
2023-07-17T12:22:12Z
dc.date.available
2023-07-17T12:22:12Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/40113
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-39835
dc.description.abstract
Transcriptional corepressors of the Topless (TPL) family regulate plant hormone and immunity signaling. The lack of a genome-wide profile of their chromatin associations limits understanding of the TPL family roles in transcriptional regulation.
Chromatin immunoprecipitation with sequencing (ChIP-Seq) was performed on Arabidopsis thaliana lines expressing GFP-tagged Topless-related 1 (TPR1-GFP) with and without constitutive immunity via Enhanced Disease Susceptibility 1 (EDS1). RNA-Seq profiling of the TPR1-GFP lines and pathogen-infected tpl/tpr mutants, combined with measuring immunity, growth, and physiological parameters was employed to investigate TPL/TPR roles in immunity and defense homeostasis.
TPR1 was enriched at promoter regions of c. 1400 genes and c. 10% of the detected binding required EDS1 immunity signaling. In a tpr1 tpl tpr4 (t3) mutant, resistance to bacteria was slightly compromised, and defense-related transcriptional reprogramming was weakly reduced or enhanced, respectively, at early (< 1 h) and late 24 h stages of bacterial infection. The t3 plants challenged with bacteria or pathogen-associated molecular pattern nlp24 displayed photosystem II dysfunctions. Also, t3 plants were hypersensitive to phytocytokine pep1 at the level of root growth inhibition. Transgenic expression of TPR1 rescued these t3 physiological defects.
We propose that TPR1 and TPL family proteins function in Arabidopsis to reduce detrimental effects associated with activated transcriptional immunity.
en
dc.format.extent
16 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc/4.0/
dc.subject
Topless-related 1
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::580 Pflanzen (Botanik)::580 Pflanzen (Botanik)
dc.title
Arabidopsis Topless-related 1 mitigates physiological damage and growth penalties of induced immunity
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1111/nph.19054
dcterms.bibliographicCitation.journaltitle
New Phytologist
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.pagestart
1404
dcterms.bibliographicCitation.pageend
1419
dcterms.bibliographicCitation.volume
239
dcterms.bibliographicCitation.url
https://doi.org/10.1111/nph.19054
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Biologie
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refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1469-8137
refubium.resourceType.provider
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