dc.contributor.author
Mäusle, Sarah M.
dc.contributor.author
Agarwala, Neva
dc.contributor.author
Eichmann, Viktor G.
dc.contributor.author
Dau, Holger
dc.contributor.author
Nürnberg, Dennis J.
dc.contributor.author
Hastings, Gary
dc.date.accessioned
2024-04-08T06:43:55Z
dc.date.available
2024-04-08T06:43:55Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/40099
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-39821
dc.description.abstract
Microsecond time-resolved step-scan FTIR difference spectroscopy was used to study photosystem I (PSI) from Thermosynechococcus vestitus BP-1 (T. vestitus, formerly known as T. elongatus) at 77 K. In addition, photoaccumulated (P700+–P700) FTIR difference spectra were obtained at both 77 and 293 K. The FTIR difference spectra are presented here for the first time. To extend upon these FTIR studies nanosecond time-resolved infrared difference spectroscopy was also used to study PSI from T. vestitus at 296 K. Nanosecond infrared spectroscopy has never been used to study PSI samples at physiological temperatures, and here it is shown that such an approach has great value as it allows a direct probe of electron transfer down both branches in PSI. In PSI at 296 K, the infrared flash-induced absorption changes indicate electron transfer down the B- and A-branches is characterized by time constants of 33 and 364 ns, respectively, in good agreement with visible spectroscopy studies. These time constants are associated with forward electron transfer from A1– to FX on the B- and A-branches, respectively. At several infrared wavelengths flash-induced absorption changes at 296 K recover in tens to hundreds of milliseconds. The dominant decay phase is characterized by a lifetime of 128 ms. These millisecond changes are assigned to radical pair recombination reactions, with the changes being associated primarily with P700+ rereduction. This conclusion follows from the observation that the millisecond infrared spectrum is very similar to the photoaccumulated (P700+–P700) FTIR difference spectrum.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Photosynthesis
en
dc.subject
Photosystem I
en
dc.subject
Thermosynechococcus vestitus BP-1
en
dc.subject
Nanosecond time-resolved infrared spectroscopy
en
dc.subject
Electron transfer
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
dc.title
Nanosecond time-resolved infrared spectroscopy for the study of electron transfer in photosystem I
dc.type
Wissenschaftlicher Artikel
dc.identifier.sepid
97627
dcterms.bibliographicCitation.doi
10.1007/s11120-023-01035-9
dcterms.bibliographicCitation.journaltitle
Photosynthesis Research
dcterms.bibliographicCitation.number
2-3
dcterms.bibliographicCitation.pagestart
229
dcterms.bibliographicCitation.pageend
239
dcterms.bibliographicCitation.volume
159
dcterms.bibliographicCitation.url
https://doi.org/10.1007/s11120-023-01035-9
refubium.affiliation
Physik
refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1573-5079