dc.contributor.author
Rashid, Usman
dc.contributor.author
Becker, Sandra K.
dc.contributor.author
Sponder, Gerhard
dc.contributor.author
Trappe, Susanne
dc.contributor.author
Sandhu, Mansur A.
dc.contributor.author
Aschenbach, Jörg R.
dc.date.accessioned
2023-06-05T14:56:04Z
dc.date.available
2023-06-05T14:56:04Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/39739
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-39457
dc.description.abstract
Efficient coordination between Mg2+ and vitamin D maintains adequate Ca2+ levels during lactation. This study explored the possible interaction between Mg2+ (0.3, 0.8, and 3 mM) and 1,25-dihydroxyvitamin D3 (1,25D; 0.05 and 5 nM) during osteogenesis using bovine mesenchymal stem cells. After 21 days, differentiated osteocytes were subjected to OsteoImage analysis, alkaline phosphatase (ALP) activity measurements, and immunocytochemistry of NT5E, ENG (endoglin), SP7 (osterix), SPP1 (osteopontin), and the BGLAP gene product osteocalcin. The mRNA expression of NT5E, THY1, ENG, SP7, BGLAP, CYP24A1, VDR, SLC41A1, SLC41A2, SLC41A3, TRPM6, TRPM7, and NIPA1 was also assessed. Reducing the Mg2+ concentration in the medium increased the accumulation of mineral hydroxyapatite and ALP activity. There was no change in the immunocytochemical localization of stem cell markers. Expression of CYP24A1 was higher in all groups receiving 5 nM 1,25D. There were tendencies for higher mRNA abundance of THY1, BGLAP, and NIPA1 in cells receiving 0.3 mM Mg2+ and 5 nM 1,25D. In conclusion, low levels of Mg2+ greatly enhanced the deposition of bone hydroxyapatite matrix. The effect of Mg2+ was not modulated by 1,25D, although the expression of certain genes (including BGLAP) tended to be increased by the combination of low Mg2+ and high 1,25D concentrations.
en
dc.format.extent
13 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Low Magnesium Concentration Enforces Bone Calcium Deposition Irrespective of 1,25-Dihydroxyvitamin D3 Concentration
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
8679
dcterms.bibliographicCitation.doi
10.3390/ijms24108679
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
10
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
24
dcterms.bibliographicCitation.url
https://doi.org/10.3390/ijms24108679
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Physiologie
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
de
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1422-0067