dc.contributor.author
Goekeri, Cengiz
dc.contributor.author
Pennitz, Peter
dc.contributor.author
Groenewald, Wibke
dc.contributor.author
Behrendt, Ulrike
dc.contributor.author
Kirsten, Holger
dc.contributor.author
Zobel, Christian M.
dc.contributor.author
Berger, Sarah
dc.contributor.author
Heinz, Gitta A.
dc.contributor.author
Mashreghi, Mir-Farzin
dc.contributor.author
Wienhold, Sandra-Maria
dc.contributor.author
Dietert, Kristina
dc.contributor.author
Dorhoi, Anca
dc.contributor.author
Gruber, Achim D.
dc.contributor.author
Scholz, Markus
dc.contributor.author
Rohde, Gernot
dc.contributor.author
Suttorp, Norbert
dc.contributor.author
CAPNETZ Study Group
dc.contributor.author
Witzenrath, Martin
dc.contributor.author
Nouailles, Geraldine
dc.date.accessioned
2023-05-30T13:39:50Z
dc.date.available
2023-05-30T13:39:50Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/39608
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-39326
dc.description.abstract
Community-acquired pneumonia remains a major contributor to global communicable disease-mediated mortality. Neutrophils play a leading role in trying to contain bacterial lung infection, but they also drive detrimental pulmonary inflammation, when dysregulated. Here we aimed at understanding the role of microRNA-223 in orchestrating pulmonary inflammation during pneumococcal pneumonia. Serum microRNA-223 was measured in patients with pneumococcal pneumonia and in healthy subjects. Pulmonary inflammation in wild-type and microRNA-223-knockout mice was assessed in terms of disease course, histopathology, cellular recruitment and evaluation of inflammatory protein and gene signatures following pneumococcal infection. Low levels of serum microRNA-223 correlated with increased disease severity in pneumococcal pneumonia patients. Prolonged neutrophilic influx into the lungs and alveolar spaces was detected in pneumococci-infected microRNA-223-knockout mice, possibly accounting for aggravated histopathology and acute lung injury. Expression of microRNA-223 in wild-type mice was induced by pneumococcal infection in a time-dependent manner in whole lungs and lung neutrophils. Single-cell transcriptome analyses of murine lungs revealed a unique profile of antimicrobial and cellular maturation genes that are dysregulated in neutrophils lacking microRNA-223. Taken together, low levels of microRNA-223 in human pneumonia patient serum were associated with increased disease severity, whilst its absence provoked dysregulation of the neutrophil transcriptome in murine pneumococcal pneumonia.
en
dc.format.extent
22 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
microRNA-223
en
dc.subject
Streptococcus pneumoniae
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
MicroRNA-223 Dampens Pulmonary Inflammation during Pneumococcal Pneumonia
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
959
dcterms.bibliographicCitation.doi
10.3390/cells12060959
dcterms.bibliographicCitation.journaltitle
Cells
dcterms.bibliographicCitation.number
6
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
12
dcterms.bibliographicCitation.url
https://doi.org/10.3390/cells12060959
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Tierpathologie
refubium.affiliation.other
Tiermedizinisches Zentrum für Resistenzforschung (TZR)
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2073-4409