dc.contributor.author
Gakuya, Francis
dc.contributor.author
Akoko, James
dc.contributor.author
Wambua, Lillian
dc.contributor.author
Nyamota, Richard
dc.contributor.author
Ronoh, Bernard
dc.contributor.author
Lekolool, Isaac
dc.contributor.author
Mwatondo, Athman
dc.contributor.author
Muturi, Mathew
dc.contributor.author
Ouma, Collins
dc.contributor.author
Nthiwa, Daniel
dc.date.accessioned
2023-05-25T11:13:30Z
dc.date.available
2023-05-25T11:13:30Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/39568
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-39286
dc.description.abstract
Background
Co-infection, especially with pathogens of dissimilar genetic makeup, may result in a more devastating impact on the host. Investigations on co-infection with neglected zoonotic pathogens in wildlife are necessary to inform appropriate prevention and control strategies to reduce disease burden in wildlife and the potential transmission of these pathogens between wildlife, livestock and humans. This study assessed co-exposure of various Kenyan wildflife species with Brucella spp, Coxiella burnetii and Rift Valley fever virus (RVFV).
Methodology
A total of 363 sera from 16 different wildlife species, most of them (92.6%) herbivores, were analysed by Enzyme-linked immunosorbent assay (ELISA) for IgG antibodies against Brucella spp, C. burnetii and RVFV. Further, 280 of these were tested by PCR to identify Brucella species.
Results
Of the 16 wildlife species tested, 15 (93.8%) were seropositive for at least one of the pathogens. Mean seropositivities were 18.9% (95% CI: 15.0–23.3) for RVFV, 13.7% (95% CI: 10.3–17.7) for Brucella spp and 9.1% (95% CI: 6.3–12.5) for C. burnetii. Buffaloes (n = 269) had higher seropositivity for Brucella spp. (17.1%, 95% CI: 13.0–21.7%) and RVFV (23.4%, 95% CI: 18.6–28.6%), while giraffes (n = 36) had the highest seropositivity for C. burnetii (44.4%, 95% CI: 27.9–61.9%). Importantly, 23 of the 93 (24.7%) animals positive for at least one pathogen were co-exposed, with 25.4% (18/71) of the positive buffaloes positive for brucellosis and RVFV. On molecular analysis, Brucella DNA was detected in 46 (19.5%, CI: 14.9–24.7) samples, with 4 (8.6%, 95% CI: 2.2–15.8) being identified as B. melitensis. The Fisher’s Exact test indicated that seropositivity varied significantly within the different animal families, with Brucella (p = 0.013), C. burnetii (p = <0.001) and RVFV (p = 0.007). Location was also significantly associated (p = <0.001) with Brucella spp. and C. burnetii seropositivities.
Conclusion
Of ~20% of Kenyan wildlife that are seropositive for Brucella spp, C. burnetii and RVFV, almost 25% indicate co-infections with the three pathogens, particularly with Brucella spp and RVFV.
en
dc.format.extent
14 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Animal pathogens
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Evidence of co-exposure with Brucella spp, Coxiella burnetii, and Rift Valley fever virus among various species of wildlife in Kenya
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e0010596
dcterms.bibliographicCitation.doi
10.1371/journal.pntd.0010596
dcterms.bibliographicCitation.journaltitle
PLOS Neglected Tropical Diseases
dcterms.bibliographicCitation.number
8
dcterms.bibliographicCitation.volume
16
dcterms.bibliographicCitation.url
https://doi.org/10.1371/journal.pntd.0010596
refubium.affiliation
Veterinärmedizin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1935-2735
refubium.resourceType.provider
WoS-Alert