dc.contributor.author
Guitton-Spassky, Tiffany
dc.contributor.author
Junge, Florian
dc.contributor.author
Singh, Abhishek Kumar
dc.contributor.author
Schade, Boris
dc.contributor.author
Achazi, Katharina
dc.contributor.author
Maglione, Marta
dc.contributor.author
Sigrist, Stephan
dc.contributor.author
Rashmi, Rashmi
dc.contributor.author
Haag, Rainer
dc.date.accessioned
2023-05-22T13:02:02Z
dc.date.available
2023-05-22T13:02:02Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/39407
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-39124
dc.description.abstract
Enzymes are more selective and efficient than synthetic catalysts but are limited by difficult recycling. This is overcome by immobilisation, namely through encapsulation, with the main drawback of this method being slow diffusion of products and reactants, resulting in effectively lowered enzyme activity. Fluorinated dendritic amphiphiles were reported to self-assemble into regularly perforated bilayer vesicles, so-called “stomatosomes”. It was proposed that they could be promising novel reaction vessels due to their increased porosity while retaining larger biomolecules at the same time. Amphiphiles were synthesised and their aggregation was analysed by cryogenic transmission electron microscopy (cryo-TEM) and dynamic light scattering (DLS) in buffered conditions necessary for enzyme encapsulation. Urease and albumin were encapsulated using the thin-film hydration method and investigated by confocal and time-gated stimulated emission depletion microscopy (gSTED). Their release was then used to probe the selective retention of cargo by stomatosomes. Free and encapsulated enzyme activity were compared and their capacity to be reused was evaluated using the Berthelot method. Urease was successfully encapsulated, did not leak out at room temperature, and showed better activity in perforated vesicles than in closed vesicles without perforations. Encapsulated enzyme could be reused with retained activity over 8 cycles using centrifugation, while free enzyme had to be filtrated. These results show that stomatosomes may be used in enzyme immobilisation applications and present advantages over closed vesicles or free enzyme.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Fluorinated dendritic amphiphiles
en
dc.subject
stomatosome aggregates
en
dc.subject
enzyme encapsulation
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.title
Fluorinated dendritic amphiphiles, their stomatosome aggregates and application in enzyme encapsulation
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1039/D3NR00493G
dcterms.bibliographicCitation.journaltitle
Nanoscale
dcterms.bibliographicCitation.number
17
dcterms.bibliographicCitation.pagestart
7781
dcterms.bibliographicCitation.pageend
7791
dcterms.bibliographicCitation.volume
15
dcterms.bibliographicCitation.url
https://doi.org/10.1039/D3NR00493G
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie

refubium.affiliation.other
Institut für Biologie

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2040-3372
refubium.resourceType.provider
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