dc.contributor.author
Faragli, A.
dc.contributor.author
Merz, Sophie
dc.contributor.author
Muzio, F. P. Lo
dc.contributor.author
Doeblin, P.
dc.contributor.author
Tanacli, R.
dc.contributor.author
Kolp, C.
dc.contributor.author
Abawi, D.
dc.contributor.author
Ötvös, J.
dc.contributor.author
Stehning, C.
dc.contributor.author
Schnackenburg, B.
dc.contributor.author
Pieske, B.
dc.contributor.author
Post, H.
dc.contributor.author
Klopfleisch, Robert
dc.contributor.author
Alogna, A.
dc.contributor.author
Kelle, S.
dc.date.accessioned
2023-05-19T14:08:51Z
dc.date.available
2023-05-19T14:08:51Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/39356
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-39073
dc.description.abstract
Right ventricular biopsy represents the gold standard for the assessment of myocardial fibrosis and collagen content. This invasive technique, however, is accompanied by perioperative complications and poor reproducibility. Extracellular volume (ECV) measured through cardiovascular magnetic resonance (CMR) has emerged as a valid surrogate method to assess fibrosis non-invasively. Nonetheless, ECV provides an overestimation of collagen concentration since it also considers interstitial space. Our study aims to investigate the feasibility of estimating total collagen volume (TCV) through CMR by comparing it with the TCV measured at histology. Seven healthy Landrace pigs were acutely instrumented closed-chest and transported to the MRI facility for measurements. For each protocol, CMR imaging at 3T was acquired. MEDIS software was used to analyze T1 mapping and ECV for both the left ventricular myocardium (LVmyo) and left ventricular septum (LVseptum). ECV was then used to estimate TCVCMR at LVmyo and LVseptum following previously published formulas. Tissues were prepared following an established protocol and stained with picrosirius red to analyze the TCVhisto in LVmyo and LVseptum. TCV measured at LVmyo and LVseptum with both histology (8 ± 5 ml and 7 ± 3 ml, respectively) and T1-Mapping (9 ± 5 ml and 8 ± 6 ml, respectively) did not show any regional differences. TCVhisto and TCVCMR showed a good level of data agreement by Bland–Altman analysis. Estimation of TCV through CMR may be a promising way to non-invasively assess myocardial collagen content and may be useful to track disease progression or treatment response.
en
dc.format.extent
9 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Cardiovascular magnetic resonance
en
dc.subject
Total collagen volume
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Estimation of total collagen volume: a T1 mapping versus histological comparison study in healthy Landrace pigs
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1007/s10554-020-01881-x
dcterms.bibliographicCitation.journaltitle
The International Journal of Cardiovascular Imaging
dcterms.bibliographicCitation.number
9
dcterms.bibliographicCitation.pagestart
1761
dcterms.bibliographicCitation.pageend
1769
dcterms.bibliographicCitation.volume
36
dcterms.bibliographicCitation.url
https://doi.org/10.1007/s10554-020-01881-x
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Tierpathologie

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1569-5794
dcterms.isPartOf.eissn
1573-0743
refubium.resourceType.provider
DeepGreen