dc.contributor.author
Mating, Moritz
dc.contributor.author
Sharbati, Soroush
dc.contributor.author
Einspanier, Ralf
dc.date.accessioned
2023-04-20T09:49:30Z
dc.date.available
2023-04-20T09:49:30Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/39002
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-38718
dc.description.abstract
Honeybees are important managed pollinators that perform important ecological and economic functions. In recent decades, the obligate ectoparasite Varroa destructor severely affected survival of honeybees as it either feeds on hemolymph and fat bodies or acts as a vector for viruses. A common treatment against the varroa mite is formic acid, which has been used for many years by beekeepers. This treatment is known to be effective, but the therapeutic index is very narrow. Many beekeepers report negative effects of formic acid on bees, which include damage to brood, worker bee mortality, and queen loss. Little is yet known about the molecular mechanisms of formic acid detoxification in honeybees. Our previous study shows the upregulation of predicted 10-formyl tetrahydrofolate dehydrogenase (10-FTHFDH) transcripts in honeybees exposed to formic acid. Here, the predicted honeybee-specific 10-FTHFDH is recombinantly expressed, and its hydrolase and dehydrogenase activities are investigated. As a result, the enzyme shows similar dehydrogenase activity in comparison to known 10-FTHFDHs. This study provides further knowledge to better understand the detoxification mechanisms of formic acid in Apis mellifera.
en
dc.format.extent
9 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Apis mellifera
en
dc.subject
Varroa destructor
en
dc.subject
detoxification
en
dc.subject
honey bee (Apis mellifera L.)
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
A Detoxification Enzyme for Apis mellifera Newly Characterized by Recombinant Expression: 10-Formyl Tetrahydrofolate Dehydrogenase
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
829869
dcterms.bibliographicCitation.doi
10.3389/finsc.2022.829869
dcterms.bibliographicCitation.journaltitle
Frontiers in Insect Science
dcterms.bibliographicCitation.originalpublishername
Frontiers Media S.A.
dcterms.bibliographicCitation.volume
2 (2022)
dcterms.bibliographicCitation.url
https://doi.org/10.3389/finsc.2022.829869
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Biochemie
refubium.note.author
We acknowledge support by the Open Access Publication Initiative of Freie Universität Berlin.
en
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2673-8600
refubium.resourceType.provider
DeepGreen