dc.contributor.author
Liu, Tong
dc.contributor.author
Dogan, Inci
dc.contributor.author
Rothe, Michael
dc.contributor.author
Reichardt, Jana
dc.contributor.author
Knauf, Felix
dc.contributor.author
Gollasch, Maik
dc.contributor.author
Luft, Friedrich C.
dc.contributor.author
Gollasch, Benjamin
dc.date.accessioned
2023-03-23T16:32:28Z
dc.date.available
2023-03-23T16:32:28Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/38549
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-38265
dc.description.abstract
Long-chain fatty acids (LCFAs) serve as energy sources, components of cell membranes, and precursors for signaling molecules. Uremia alters LCFA metabolism so that the risk of cardiovascular events in chronic kidney disease (CKD) is increased. End-stage renal disease (ESRD) patients undergoing dialysis are particularly affected and their hemodialysis (HD) treatment could influence blood LCFA bioaccumulation and transformation. We investigated blood LCFA in HD patients and studied LCFA profiles in vivo by analyzing arterio-venous (A-V) LFCA differences in upper limbs. We collected arterial and venous blood samples from 12 ESRD patients, before and after HD, and analyzed total LCFA levels in red blood cells (RBCs) and plasma by LC-MS/MS tandem mass spectrometry. We observed that differences in arterial and venous LFCA contents within RBCs (RBC LCFA A-V differences) were affected by HD treatment. Numerous saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) n-6 showed negative A-V differences, accumulated during peripheral tissue perfusion of the upper limbs, in RBCs before HD. HD reduced these differences. The omega-3 quotient in the erythrocyte membranes was not affected by HD in either arterial or venous blood. Our data demonstrate that A-V differences in fatty acids status of LCFA are present and active in mature erythrocytes and their bioaccumulation is sensitive to single HD treatment.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
erythrocytes
en
dc.subject
chronic kidney disease
en
dc.subject
hemodialysis
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Bioaccumulation of Blood Long-Chain Fatty Acids during Hemodialysis
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
269
dcterms.bibliographicCitation.doi
10.3390/metabo12030269
dcterms.bibliographicCitation.journaltitle
Metabolites
dcterms.bibliographicCitation.number
3
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
12
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
35323712
dcterms.isPartOf.eissn
2218-1989