dc.contributor.author
Lowenstein, Elijah David
dc.date.accessioned
2023-03-10T11:51:16Z
dc.date.available
2023-03-10T11:51:16Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/38267
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-37986
dc.description.abstract
Sensory neurons of the vagus nerve monitor distention, stretch and nutrients in the gastrointestinal tract, and major efforts are underway to assign physiological functions to the many distinct subtypes of vagal sensory neurons. Here, we used genetically guided anatomical tracing, optogenetics and electrophysiology to identify and characterize three vagal sensory neuronal subtypes expressing Prox2 and Runx3 in mice. We show that these neuronal subtypes function as putative mechanoreceptors. They innervate the esophagus and stomach where they display regionalized innervation patterns, as well as other organs. The electrophysiological analysis of Prox2/Runx3 neurons innervating the esophagus showed that they are all low threshold mechanoreceptors, but possess different adaptation properties. Lastly, genetic ablation of Prox2 and Runx3 neurons demonstrated their essential roles for esophageal peristalsis and swallowing in freely behaving mice. Our work reveals the identity and function of the vagal neurons that provide mechanosensory feedback from the esophagus to the brain, and might lead to better understanding and treatment of esophageal motility disorders.
en
dc.format.extent
117 Seiten
dc.rights.uri
http://www.fu-berlin.de/sites/refubium/rechtliches/Nutzungsbedingungen
dc.subject
vagal afferents
en
dc.subject
single cell RNA sequencing
en
dc.subject
mechanoreceptors
en
dc.subject
mechanosensation
en
dc.subject.ddc
500 Natural sciences and mathematics::570 Life sciences::570 Life sciences
dc.title
Connecting the esophagus to the brain
dc.contributor.gender
male
dc.contributor.firstReferee
Birchmeier, Carmen
dc.contributor.furtherReferee
Hiesinger, Robin
dc.date.accepted
2023-02-15
dc.identifier.urn
urn:nbn:de:kobv:188-refubium-38267-2
dc.title.subtitle
examining the role of Prox2 and Runx3 vagal sensory neurons in swallowing
dc.title.translated
Verbindung der Speiseröhre mit dem Gehirn – Untersuchung der Rolle der vagalen sensorischen Neuronen Prox2 und Runx3 beim Schlucken
ger
refubium.affiliation
Biologie, Chemie, Pharmazie
dcterms.accessRights.dnb
free
dcterms.accessRights.openaire
open access
dcterms.accessRights.proquest
accept