dc.contributor.author
Schubert, Luiz
dc.contributor.author
Langner, Pit
dc.contributor.author
Ehrenberg, David
dc.contributor.author
Lorenz-Fonfria, Victor A.
dc.contributor.author
Heberle, Joachim
dc.date.accessioned
2023-03-09T08:43:03Z
dc.date.available
2023-03-09T08:43:03Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/38083
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-37796
dc.description.abstract
Mid-IR spectroscopy is a powerful and label-free technique to investigate protein reactions. In this study, we use quantum-cascade-laser-based dual-comb spectroscopy to probe protein conformational changes and protonation events by a single-shot experiment. By using a well-characterized membrane protein, bacteriorhodopsin, we provide a comparison between dual-comb spectroscopy and our homebuilt tunable quantum cascade laser (QCL)-based scanning spectrometer as tools to monitor irreversible reactions with high time resolution. In conclusion, QCL-based infrared spectroscopy is demonstrated to be feasible for tracing functionally relevant protein structural changes and proton translocations by single-shot experiments. Thus, we envisage a bright future for applications of this technology for monitoring the kinetics of irreversible reactions as in (bio-)chemical transformations.
en
dc.format.extent
9 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Signal-to-noise ratio
en
dc.subject
Interferometry
en
dc.subject
Kinetic isotope effects
en
dc.subject
Frequency combs
en
dc.subject
Fourier transform spectroscopy
en
dc.subject
Infrared spectroscopy
en
dc.subject
Macromolecules
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik::539 Moderne Physik
dc.title
Protein conformational changes and protonation dynamics probed by a single shot using quantum-cascade-laser-based IR spectroscopy
dc.type
Wissenschaftlicher Artikel
dc.identifier.sepid
90989
dcterms.bibliographicCitation.articlenumber
204201
dcterms.bibliographicCitation.doi
10.1063/5.0088526
dcterms.bibliographicCitation.journaltitle
The Journal of Chemical Physics
dcterms.bibliographicCitation.number
20
dcterms.bibliographicCitation.originalpublishername
American Institute of Physics
dcterms.bibliographicCitation.originalpublisherplace
Melville, NY
dcterms.bibliographicCitation.volume
156 (2022)
dcterms.bibliographicCitation.url
https://aip.scitation.org/doi/10.1063/5.0088526
refubium.affiliation
Physik
refubium.affiliation.other
Institut für Experimentalphysik
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
0021-9606
dcterms.isPartOf.eissn
1089-7690