dc.contributor.author
Wolf, Alexander
dc.contributor.author
Volz-Rakebrand, Pierre
dc.contributor.author
Balke, Jens
dc.contributor.author
Alexiev, Ulrike
dc.date.accessioned
2023-05-19T10:33:17Z
dc.date.available
2023-05-19T10:33:17Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/37815
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-37528
dc.description.abstract
The rapid development of microscopic techniques over the past decades enables the establishment of single molecule fluorescence imaging as a powerful tool in biological and biomedical sciences. Single molecule fluorescence imaging allows to study the chemical, physicochemical, and biological properties of target molecules or particles by tracking their molecular position in the biological environment and determining their dynamic behavior. However, the precise determination of particle distribution and diffusivities is often challenging due to high molecule/particle densities, fast diffusion, and photobleaching/blinking of the fluorophore. A novel, accurate, and fast statistical analysis tool, Diffusion Analysis of NAnoscopic Ensembles (DANAE), that solves all these obstacles is introduced. DANAE requires no approximations or any a priori input regarding unknown system-inherent parameters, such as background distributions; a requirement that is vitally important when studying the behavior of molecules/particles in living cells. The superiority of DANAE with various data from simulations is demonstrated. As experimental applications of DANAE, membrane receptor diffusion in its natural membrane environment, and cargo mobility/distribution within nanostructured lipid nanoparticles are presented. Finally, the method is extended to two-color channel fluorescence microscopy.
en
dc.format.extent
9 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
molecular interactions
en
dc.subject
nanocarriers
en
dc.subject
nanoscopic diffusion
en
dc.subject
single molecule fluorescence
en
dc.subject
tracking-free
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
dc.title
Diffusion Analysis of NAnoscopic Ensembles: A Tracking-Free Diffusivity Analysis for Nanoscopic Ensembles in Biological Samples and Nanotechnology
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2206722
dcterms.bibliographicCitation.doi
10.1002/smll.202206722
dcterms.bibliographicCitation.journaltitle
Small
dcterms.bibliographicCitation.number
16
dcterms.bibliographicCitation.volume
19
dcterms.bibliographicCitation.url
https://doi.org/10.1002/smll.202206722
refubium.affiliation
Physik
refubium.funding
DEAL Wiley
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1613-6829