dc.contributor.author
Mating, Moritz
dc.contributor.author
Zou, Ye
dc.contributor.author
Sharbati, Soroush
dc.contributor.author
Einspanier, Ralf
dc.date.accessioned
2023-01-12T10:02:15Z
dc.date.available
2023-01-12T10:02:15Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/37571
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-37285
dc.description.abstract
Honey bees are important managed pollinators that fulfill important ecological and economic functions. In recent decades, the obligate ectoparasite Varroa destructor severely affected the survival of honey bees, as it weakened them by different means. A common treatment against V. destructor is formic acid fumigation, which has been used for decades by beekeepers across the world. This treatment is known to be effective, but many beekeepers report adverse effects of formic acid on bees, which include damage to the brood, worker bee mortality, and queen loss. Little is known about the molecular mechanisms of formic acid detoxification in honey bees. Recently, we reported upregulation of the bee enzyme, 10-formyl-THFDH, under formic acid fumigation. Here, the active site of this enzyme is characterized by an interdisciplinary approach combining homology modeling and protein mutagenesis. In addition, the limitations of the 3D protein structure prediction program AlphaFold2 are shown in regard to docking studies. This study provides a more thorough understanding of the molecular detoxification mechanisms of formic acid in Apis mellifera.
en
dc.format.extent
13 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Apis mellifera
en
dc.subject
10-formyl-THFDH
en
dc.subject
detoxification
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::577 Ökologie
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::577 Ökologie
dc.title
The Active Site of the Enzyme 10-Formyl-THFDH in the Honey Bee Apis mellifera - A Key Player in Formic Acid Detoxification
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
354
dcterms.bibliographicCitation.doi
10.3390/ijms24010354
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
24
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Biochemie

refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
de
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1422-0067